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dc.contributor.author
Vetter, Douglas E.
dc.contributor.author
Katz, Eleonora
dc.contributor.author
Maison, Stéphane F.
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Taranda, Julian
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Turcan, Sevin
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Ballestero, Jimena Andrea
dc.contributor.author
Liberman, M. Charles
dc.contributor.author
Elgoyhen, Ana Belen
dc.contributor.author
Boulter, Jim
dc.date.available
2019-07-22T20:23:03Z
dc.date.issued
2007-12
dc.identifier.citation
Vetter, Douglas E.; Katz, Eleonora; Maison, Stéphane F.; Taranda, Julian; Turcan, Sevin; et al.; The α10 nicotinic acetylcholine receptor subunit is required for normal synaptic function and integrity of the olivocochlear system; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 104; 51; 12-2007; 20594-20599
dc.identifier.issn
0027-8424
dc.identifier.uri
http://hdl.handle.net/11336/80009
dc.description.abstract
Although homomeric channels assembled from the α9 nicotinic acetylcholine receptor (nAChR) subunit are functional in vitro, electrophysiological, anatomical, and molecular data suggest that native cholinergic olivocochlear function is mediated via heteromeric nAChRs composed of both α9 and α10 subunits. To gain insight into α10 subunit function in vivo, we examined olivocochlear innervation and function in α10 null-mutant mice. Electrophysiological recordings from postnatal (P) days P8-9 inner hair cells revealed ACh-gated currents in α10 +/+ and α10+/- mice, with no detectable responses to ACh in α10+/+ mice. In contrast, a proportion of α10-/- outer hair cells showed small ACh-evoked currents. In α10-/- mutant mice, olivocochlear fiber stimulation failed to suppress distortion products, suggesting that the residual α9 homomeric nAChRs expressed by outer hair cells are unable to transduce efferent signals in vivo. Finally, α10-/- mice exhibit both an abnormal olivocochlear morphology and innervation to outer hair cells and a highly disorganized efferent innervation to the inner hair cell region. Our results demonstrate that α9-/- and α10-/- mice have overlapping but nonidentical phenotypes. Moreover, α10 nAChR subunits are required for normal olivocochlear activity because α9 homomeric nAChRs do not support maintenance of normal olivocochlear innervation or function in α10-/- mutant mice.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
National Academy of Sciences
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Cochlea
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Electrophysiology
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Inner Hair Cells
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Outer Hair Cells
dc.subject.classification
Neurociencias
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Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
The α10 nicotinic acetylcholine receptor subunit is required for normal synaptic function and integrity of the olivocochlear system
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-07-15T13:54:00Z
dc.journal.volume
104
dc.journal.number
51
dc.journal.pagination
20594-20599
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Washington
dc.description.fil
Fil: Vetter, Douglas E.. Tufts University School Of Medicine; Estados Unidos
dc.description.fil
Fil: Katz, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires; Argentina
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Fil: Maison, Stéphane F.. Harvard Medical School; Estados Unidos. Tufts University School Of Medicine; Estados Unidos. Massachusetts Eye And Ear Infirmary; Estados Unidos
dc.description.fil
Fil: Taranda, Julian. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Tufts University School Of Medicine; Estados Unidos
dc.description.fil
Fil: Turcan, Sevin. Tufts University School Of Medicine; Estados Unidos
dc.description.fil
Fil: Ballestero, Jimena Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
dc.description.fil
Fil: Liberman, M. Charles. Universidad de Buenos Aires; Argentina
dc.description.fil
Fil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires; Argentina
dc.description.fil
Fil: Boulter, Jim. University of California at Los Angeles. School of Medicine; Estados Unidos
dc.journal.title
Proceedings of the National Academy of Sciences of The United States of America
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://www.pnas.org/content/104/51/20594
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1073/pnas.0708545105
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2154476/
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