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dc.contributor.author
Hamdani, Omar
dc.contributor.author
Dhillon, Namrita
dc.contributor.author
Hsieh, Tsung-Han S.
dc.contributor.author
Fujita, Takahiro
dc.contributor.author
Ocampo, Josefina
dc.contributor.author
Kirkland, Jacob G.
dc.contributor.author
Lawrimore, Josh
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Kobayashi, Tetsuya J.
dc.contributor.author
Friedman, Brandon
dc.contributor.author
Fulton, Derek
dc.contributor.author
Wu, Kenneth Y.
dc.contributor.author
Chereji, Razvan V.
dc.contributor.author
Oki, Masaya
dc.contributor.author
Bloom, Kerry
dc.contributor.author
Clark, David
dc.contributor.author
Rando, Oliver J.
dc.contributor.author
Kamakaka, Rohinton T.
dc.date.available
2019-07-19T18:52:30Z
dc.date.issued
2019-04
dc.identifier.citation
Hamdani, Omar; Dhillon, Namrita; Hsieh, Tsung-Han S.; Fujita, Takahiro; Ocampo, Josefina; et al.; TRNA genes affect chromosome structure and function via local effects; American Society for Microbiology; Molecular and Cellular Biology; 39; 8; 4-2019; 1-67
dc.identifier.issn
0270-7306
dc.identifier.uri
http://hdl.handle.net/11336/79920
dc.description.abstract
The genome is packaged and organized in an ordered, nonrandom manner, and specific chromatin segments contact nuclear substructures to mediate this organization. tRNA genes (tDNAs) are binding sites for transcription factors and architectural proteins and are thought to play an important role in the organization of the genome. In this study, we investigate the roles of tDNAs in genomic organization and chromosome function by editing a chromosome so that it lacked any tDNAs. Surprisingly our analyses of this tDNA-less chromosome show that loss of tDNAs does not grossly affect chromatin architecture or chromosome tethering and mobility. However, loss of tDNAs affects local nucleosome positioning and the binding of SMC proteins at these loci. The absence of tDNAs also leads to changes in centromere clustering and a reduction in the frequency of long-range HML-HMR heterochromatin clustering with concomitant effects on gene silencing. We propose that the tDNAs primarily affect local chromatin structure, which results in effects on long-range chromosome architecture.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Society for Microbiology
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Chromatin
dc.subject
Chromosome Structure
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Gene Silencing
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Nucleosome
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Saccharomyces Cerevisiae
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Smc Proteins
dc.subject
Tdna
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Otras Ciencias Biológicas
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Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
TRNA genes affect chromosome structure and function via local effects
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-07-17T13:14:50Z
dc.journal.volume
39
dc.journal.number
8
dc.journal.pagination
1-67
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Washington
dc.description.fil
Fil: Hamdani, Omar. University of California; Estados Unidos
dc.description.fil
Fil: Dhillon, Namrita. University of California; Estados Unidos
dc.description.fil
Fil: Hsieh, Tsung-Han S.. University of Massachusetts Medical School; Estados Unidos
dc.description.fil
Fil: Fujita, Takahiro. University of Fukui; Japón
dc.description.fil
Fil: Ocampo, Josefina. Eunice Kennedy Shriver National Institute of Child Health and Human Development; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
dc.description.fil
Fil: Kirkland, Jacob G.. University of California; Estados Unidos
dc.description.fil
Fil: Lawrimore, Josh. University of North Carolina at Chapel Hill; Estados Unidos
dc.description.fil
Fil: Kobayashi, Tetsuya J.. The University of Tokyo; Japón
dc.description.fil
Fil: Friedman, Brandon. University of North Carolina at Chapel Hill; Estados Unidos
dc.description.fil
Fil: Fulton, Derek. University of North Carolina at Chapel Hill; Estados Unidos
dc.description.fil
Fil: Wu, Kenneth Y.. University of California; Estados Unidos
dc.description.fil
Fil: Chereji, Razvan V.. Eunice Kennedy Shriver National Institute of Child Health and Human Development; Estados Unidos
dc.description.fil
Fil: Oki, Masaya. University of Fukui; Japón
dc.description.fil
Fil: Bloom, Kerry. University of North Carolina at Chapel Hill; Estados Unidos
dc.description.fil
Fil: Clark, David. Eunice Kennedy Shriver National Institute of Child Health and Human Development; Estados Unidos
dc.description.fil
Fil: Rando, Oliver J.. University of Massachusetts Medical School; Estados Unidos
dc.description.fil
Fil: Kamakaka, Rohinton T.. University of California; Estados Unidos
dc.journal.title
Molecular and Cellular Biology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/30718362/
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1128/MCB.00432-18
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://mcb.asm.org/content/39/8/e00432-18
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