Artículo
Cladosporium cladosporioides LPSC 1088 Produces the 1,8-Dihydroxynaphthalene-Melanin-Like Compound and Carries a Putative pks Gene
Llorente, Carla
; Bárcena, Alejandra
; Vera Bahima, José
; Saparrat, Mario Carlos Nazareno
; Arambarri, Angélica Margarita
; Rozas, Maria Fernanda
; Mirifico, Maria Virginia
; Balatti, Pedro Alberto
Fecha de publicación:
07/2012
Editorial:
Springer
Revista:
Mycopathologia
ISSN:
0301-486X
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Cladosporium cladosporioides is a dematiaceous fungus with coloured mycelia and conidia due to the presence of dark pigments. The purpose of this study was to characterize the dark pigments synthetized by Cladosporium sp. LPSC no. 1088 and also to identify the putative polyketide synthase (pks) gene that might be involved in the pigment biosynthesis. Morphological as well as molecular features like the ITS sequence confirmed that LPSC 1088 is Cladosporium cladosporioides. UV-visible, Fourier Transform Infrared (FTIR) and Electron Spin Resonance (ESR) spectroscopy analysis as well as melanin inhibitors suggest that the main dark pigment of the isolate was 1,8 dihydroxynaphthalene (DHN)-melanin-type compound. Two commercial fungicides, Difenoconazole and Chlorothalonil, inhibited fungal growth as well as increased pigmentation of the colonies suggesting that melanin might protect the fungus against chemical stress. The pigment is most probably synthetized by means of a pentaketide pathway since the sequence of a 651 bp fragment, coding for a putative polyketide synthase, is highly homologous to pks sequences from other fungi.
Palabras clave:
Cladosporium Cladosporioides
,
Dhn Melanin
,
Fungicides
,
Pks
,
Polyketide Synthase
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CCT - LA PLATA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Citación
Llorente, Carla; Bárcena, Alejandra; Vera Bahima, José; Saparrat, Mario Carlos Nazareno; Arambarri, Angélica Margarita; et al.; Cladosporium cladosporioides LPSC 1088 Produces the 1,8-Dihydroxynaphthalene-Melanin-Like Compound and Carries a Putative pks Gene; Springer; Mycopathologia; 174; 5-6; 7-2012; 397-408
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