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dc.contributor.author
Guevara, Palmira
dc.contributor.author
Dias, Manuel
dc.contributor.author
Rojas, Agustina
dc.contributor.author
Crisante, Gladys
dc.contributor.author
Abreu Blanco, Maria Teresa
dc.contributor.author
Umezawa, Eufrozina
dc.contributor.author
Vazquez, Martin Pablo
dc.contributor.author
Levin, Mariano Jorge
dc.contributor.author
Añez, Nestor
dc.contributor.author
Ramirez, Jose Luis
dc.date.available
2019-07-18T21:46:12Z
dc.date.issued
2005-01
dc.identifier.citation
Guevara, Palmira; Dias, Manuel; Rojas, Agustina; Crisante, Gladys; Abreu Blanco, Maria Teresa; et al.; Expression of Fluorescent Genes in Trypanosoma cruzi and Trypanosoma rangeli (Kinetoplastida: Trypanosomatidae): Its Application to Parasite-Vector Biology; Oxford University Press; Journal of Medical Entomology; 42; 1; 1-2005; 48-56
dc.identifier.issn
0022-2585
dc.identifier.uri
http://hdl.handle.net/11336/79880
dc.description.abstract
Two Trypanosoma cruzi-derived cloning vectors, pTREX-n and pBs:CalB1/CUB01, were used to drive the expression of green fluorescent protein (GFP) and DsRed in Trypanosoma rangeli Tejera, 1920, and Trypanosoma cruzi Chagas, 1909, isolates, respectively. Regardless of the species, group, or strain, parasites harboring the transfected constructs as either episomes or stable chromosomal integrations showed high-level expression of fluorescent proteins. Tagged flagellates of both species were used to experimentally infect Rhodnius prolixus Stal, 1953. In infected bugs, single or mixed infections of T. cruzi and T. rangeli displayed the typical cycle of each species, with no apparent interspecies interactions. In addition, infection of kidney monkey cells (LLC-MK2) with GFP-T. cruzi showed that the parasite retained its fluorescent tag while carrying out its life cycle within cultured cells. The use of GFP-tagged parasites as a tool for biological studies in experimental hosts is discussed, as is the application of this method for copopulation studies of same-host parasites
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Oxford University Press
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject.classification
Inmunología
dc.subject.classification
Medicina Básica
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Expression of Fluorescent Genes in Trypanosoma cruzi and Trypanosoma rangeli (Kinetoplastida: Trypanosomatidae): Its Application to Parasite-Vector Biology
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-07-18T13:02:07Z
dc.journal.volume
42
dc.journal.number
1
dc.journal.pagination
48-56
dc.journal.pais
Reino Unido
dc.journal.ciudad
Oxford
dc.description.fil
Fil: Guevara, Palmira. Universidad Central de Venezuela; Venezuela
dc.description.fil
Fil: Dias, Manuel. Universidad de los Andes; Colombia
dc.description.fil
Fil: Rojas, Agustina. Universidad de los Andes; Colombia
dc.description.fil
Fil: Crisante, Gladys. Universidad de los Andes; Colombia
dc.description.fil
Fil: Abreu Blanco, Maria Teresa. Universidad Central de Venezuela; Venezuela
dc.description.fil
Fil: Umezawa, Eufrozina. Universidade de Sao Paulo; Brasil
dc.description.fil
Fil: Vazquez, Martin Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
dc.description.fil
Fil: Levin, Mariano Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
dc.description.fil
Fil: Añez, Nestor. Universidad de los Andes; Colombia
dc.description.fil
Fil: Ramirez, Jose Luis. Instituto de Estudios Avanzados; Venezuela
dc.journal.title
Journal of Medical Entomology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/15691008
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1093/jmedent/42.1.48
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jme/article/42/1/48/847455
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