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dc.contributor.author
Cloutier, Monikca
dc.contributor.author
Castilla Lozano, Maria del Rocio
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dc.contributor.author
Bolduc, Nathalie
dc.contributor.author
Zelada, Alicia Mercedes
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dc.contributor.author
Martineau, Philippe
dc.contributor.author
Bouillon, Marlène
dc.contributor.author
Magee, Beatrice B.
dc.contributor.author
Di Bernardo, Maria Susana
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dc.contributor.author
Giasson, Luc
dc.contributor.author
Cantore, Maria Leonor
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dc.date.available
2019-07-17T22:21:59Z
dc.date.issued
2003-02
dc.identifier.citation
Cloutier, Monikca; Castilla Lozano, Maria del Rocio; Bolduc, Nathalie; Zelada, Alicia Mercedes; Martineau, Philippe; et al.; The two isoforms of the cAMP-dependent protein kinase catalytic subunit are involved in the control of dimorphism in the human fungal pathogen Candida albicans; Academic Press Inc Elsevier Science; Fungal Genetics And Biology; 38; 1; 2-2003; 133-141
dc.identifier.issn
1087-1845
dc.identifier.uri
http://hdl.handle.net/11336/79778
dc.description.abstract
We have cloned the Candida albicans TPK2 gene encoding a cAMP-dependent protein kinase (PKA) catalytic subunit and generated a tpk2 homozygous null mutant to assess its ability to germinate in liquid media. N-acetylglucosamine (GlcNAc)-induced germ-tube formation was attenuated in the tpk2 strain and enhanced by compounds that are known to increase the PKA activity in situ. Germination was completely blocked in the presence of the myristoylated derivative of the heat-stable PKA inhibitor (MyrPKI). These results indicate that TPK1 acts positively in regulating the morphogenetic transition in C. albicans in the absence of the TPK2 gene. We were able to identify an mRNA from this second form of PKA in both wild-type and tpk2 null mutant cells. We found that PKA activity measured in the mutant lacking the TPK2 gene was about 10% of that displayed by the wild-type. The finding that the germinative response of tpk2 null mutant to serum was severely diminished at low serum concentrations indicates that the level of PKA is an important determinant of filamentous growth at low serum concentrations. The extent of germination attained at higher serum concentrations (5%) was similar in the wild-type and in the tpk2 null mutant strains suggesting that under these conditions germination was triggered through a PKA-independent pathway.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Academic Press Inc Elsevier Science
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Camp
dc.subject
Camp-Dependent Protein Kinase
dc.subject
Candida Albicans
dc.subject
Morphogenesis
dc.subject.classification
Bioquímica y Biología Molecular
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
The two isoforms of the cAMP-dependent protein kinase catalytic subunit are involved in the control of dimorphism in the human fungal pathogen Candida albicans
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-07-16T14:13:49Z
dc.journal.volume
38
dc.journal.number
1
dc.journal.pagination
133-141
dc.journal.pais
Países Bajos
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dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Cloutier, Monikca. GREB Laval University; Canadá
dc.description.fil
Fil: Castilla Lozano, Maria del Rocio. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invetigaciones Bioquímicas y Fisiologicas; Argentina
dc.description.fil
Fil: Bolduc, Nathalie. GREB Laval University; Canadá
dc.description.fil
Fil: Zelada, Alicia Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invetigaciones Bioquímicas y Fisiologicas; Argentina
dc.description.fil
Fil: Martineau, Philippe. GREB Laval University; Canadá
dc.description.fil
Fil: Bouillon, Marlène. GREB Laval University; Canadá
dc.description.fil
Fil: Magee, Beatrice B.. University of Minnesota; Estados Unidos
dc.description.fil
Fil: Di Bernardo, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invetigaciones Bioquímicas y Fisiologicas; Argentina
dc.description.fil
Fil: Giasson, Luc. GREB Laval University; Canadá
dc.description.fil
Fil: Cantore, Maria Leonor. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invetigaciones Bioquímicas y Fisiologicas; Argentina
dc.journal.title
Fungal Genetics And Biology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/12553943
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1087184502005200
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/S1087-1845(02)00520-0
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