Artículo
Functional expression and properties of a nicotinic alpha9/5-HT3A chimeric receptor
Fecha de publicación:
10/2003
Editorial:
Lippincott Williams
Revista:
Neuroreport
ISSN:
0959-4965
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
We describe the functional properties of a nicotinic alpha9/serotonin subtype 3A (5HT3A) chimeric receptor expressed in Xenopus laevis oocytes. The chimera preserved ligand-binding properties of alpha9 and channel properties of 5HT3A. Thus, it responded to acetylcholine in a concentration-dependent manner with an EC50 of 70 microM but not to serotonin. It was blocked by methyllycaconitine, strychnine, atropine and nicotine, with the same rank order of potency as alpha9 receptors. The current-voltage relationship of currents through the alpha9/5HT3A chimera was similar to that of the 5HT3A receptors. These results are an evidence of functional coupling between the ligand-binding and the channel domains of the chimeric receptor.
Palabras clave:
Hair Cells
,
Ion Channels
,
Nicotinic Receptors
,
Serotonin Receptors
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Articulos(INGEBI)
Articulos de INST.DE INVEST.EN ING.GENETICA Y BIOL.MOLECULAR "DR. HECTOR N TORRES"
Articulos de INST.DE INVEST.EN ING.GENETICA Y BIOL.MOLECULAR "DR. HECTOR N TORRES"
Citación
Verbitsky, Miguel; Plazas, Paola Viviana; Elgoyhen, Ana Belen; Functional expression and properties of a nicotinic alpha9/5-HT3A chimeric receptor; Lippincott Williams; Neuroreport; 14; 15; 10-2003; 1931-1934
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