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dc.contributor.author
Gelman, Diego Matias  
dc.contributor.author
Noain, Daniela Maria Clara  
dc.contributor.author
Avale, Maria Elena  
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Otero Corchon, Veronica  
dc.contributor.author
Low, Malcolm J.  
dc.contributor.author
Rubinstein, Marcelo  
dc.date.available
2019-07-16T20:52:04Z  
dc.date.issued
2003-08  
dc.identifier.citation
Gelman, Diego Matias; Noain, Daniela Maria Clara; Avale, Maria Elena; Otero Corchon, Veronica; Low, Malcolm J.; et al.; Transgenic mice engineered to target Cre/LoxP-mediated DNA recombination into catecholaminergic neurons; Wiley-liss, Div John Wiley & Sons Inc; Genesis; 36; 4; 8-2003; 196-202  
dc.identifier.issn
1526-954X  
dc.identifier.uri
http://hdl.handle.net/11336/79707  
dc.description.abstract
To introduce restricted DNA recombination events into catecholaminergic neurons using the Cre/loxP technology, we generated transgenic mice carrying the Cre recombinase gene driven by a 9 kb rat tyrosine hydroxylase (TH) promoter. Immunohistochemistry performed on transgenic mouse brain sections revealed a high number of cells expressing Cre in areas where TH is normally expressed, including the olfactory bulb, hypothalamic and midbrain dopaminergic neurons, and the locus coeruleus. Double immunohistochemistry and immunofluorescence indicated that colocalization of TH and Cre is greater than 80%. Cre expression was also found in TH-positive amacrine neurons of the retina, chromaffin cells of the adrenal medulla, and sympathetic ganglia. We intercrossed TH-Cre mice with the floxed reporter strain Z/AP and observed efficient Cre-mediated recombination in all areas expressing TH, indicating that transgenic Cre is functional. Therefore, we have generated a valuable transgenic mouse strain to induce specific mutations of "floxed" genes in catecholaminergic neurons.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Wiley-liss, Div John Wiley & Sons Inc  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Catecholamine  
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Cre Recombinase  
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Dopamine  
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Loxp  
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Norepinephrine  
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Transgenic Mouse  
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Tyrosine Hydroxylase  
dc.subject.classification
Bioquímica y Biología Molecular  
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Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Transgenic mice engineered to target Cre/LoxP-mediated DNA recombination into catecholaminergic neurons  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2019-07-16T14:14:59Z  
dc.journal.volume
36  
dc.journal.number
4  
dc.journal.pagination
196-202  
dc.journal.pais
Estados Unidos  
dc.journal.ciudad
Nueva York  
dc.description.fil
Fil: Gelman, Diego Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina  
dc.description.fil
Fil: Noain, Daniela Maria Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina  
dc.description.fil
Fil: Avale, Maria Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina  
dc.description.fil
Fil: Otero Corchon, Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina  
dc.description.fil
Fil: Low, Malcolm J.. Oregon Health and Science University; Estados Unidos  
dc.description.fil
Fil: Rubinstein, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Centro de Estudios Científicos; Chile  
dc.journal.title
Genesis  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/12929090  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/gene.10217  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/gene.10217