Mostrar el registro sencillo del ítem
dc.contributor.author
Gelman, Diego Matias
dc.contributor.author
Noain, Daniela Maria Clara
dc.contributor.author
Avale, Maria Elena
dc.contributor.author
Otero Corchon, Veronica
dc.contributor.author
Low, Malcolm J.
dc.contributor.author
Rubinstein, Marcelo
dc.date.available
2019-07-16T20:52:04Z
dc.date.issued
2003-08
dc.identifier.citation
Gelman, Diego Matias; Noain, Daniela Maria Clara; Avale, Maria Elena; Otero Corchon, Veronica; Low, Malcolm J.; et al.; Transgenic mice engineered to target Cre/LoxP-mediated DNA recombination into catecholaminergic neurons; Wiley-liss, Div John Wiley & Sons Inc; Genesis; 36; 4; 8-2003; 196-202
dc.identifier.issn
1526-954X
dc.identifier.uri
http://hdl.handle.net/11336/79707
dc.description.abstract
To introduce restricted DNA recombination events into catecholaminergic neurons using the Cre/loxP technology, we generated transgenic mice carrying the Cre recombinase gene driven by a 9 kb rat tyrosine hydroxylase (TH) promoter. Immunohistochemistry performed on transgenic mouse brain sections revealed a high number of cells expressing Cre in areas where TH is normally expressed, including the olfactory bulb, hypothalamic and midbrain dopaminergic neurons, and the locus coeruleus. Double immunohistochemistry and immunofluorescence indicated that colocalization of TH and Cre is greater than 80%. Cre expression was also found in TH-positive amacrine neurons of the retina, chromaffin cells of the adrenal medulla, and sympathetic ganglia. We intercrossed TH-Cre mice with the floxed reporter strain Z/AP and observed efficient Cre-mediated recombination in all areas expressing TH, indicating that transgenic Cre is functional. Therefore, we have generated a valuable transgenic mouse strain to induce specific mutations of "floxed" genes in catecholaminergic neurons.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley-liss, Div John Wiley & Sons Inc
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Catecholamine
dc.subject
Cre Recombinase
dc.subject
Dopamine
dc.subject
Loxp
dc.subject
Norepinephrine
dc.subject
Transgenic Mouse
dc.subject
Tyrosine Hydroxylase
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Transgenic mice engineered to target Cre/LoxP-mediated DNA recombination into catecholaminergic neurons
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-07-16T14:14:59Z
dc.journal.volume
36
dc.journal.number
4
dc.journal.pagination
196-202
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Nueva York
dc.description.fil
Fil: Gelman, Diego Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
dc.description.fil
Fil: Noain, Daniela Maria Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
dc.description.fil
Fil: Avale, Maria Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
dc.description.fil
Fil: Otero Corchon, Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
dc.description.fil
Fil: Low, Malcolm J.. Oregon Health and Science University; Estados Unidos
dc.description.fil
Fil: Rubinstein, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Centro de Estudios Científicos; Chile
dc.journal.title
Genesis
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/12929090
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/gene.10217
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/gene.10217
Archivos asociados