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dc.contributor.author
Utesch, Tillmann
dc.contributor.author
Millo, Diego
dc.contributor.author
Castro, Maria Ana
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dc.contributor.author
Hildebrandt, Peter
dc.contributor.author
Zebger, Ingo
dc.contributor.author
Mroginski, Maria Andrea
dc.date.available
2016-11-03T21:43:28Z
dc.date.issued
2012-12-07
dc.identifier.citation
Utesch, Tillmann; Millo, Diego; Castro, Maria Ana; Hildebrandt, Peter ; Zebger, Ingo ; et al.; Effect of the Protonation Degree of a Self-Assembled Monolayer on the Immobilization Dynamics of a [NiFe] Hydrogenase; American Chemical Society; Langmuir; 29; 2; 7-12-2012; 673-682
dc.identifier.issn
0743-7463
dc.identifier.uri
http://hdl.handle.net/11336/7969
dc.description.abstract
Understanding the interaction and immobilization of [NiFe] hydrogenases on functionalized surfaces is important in the field of biotechnology and, in particular, for the development of biofuel cells. In this study, we investigated the adsorption behavior of the standard [NiFe] hydrogenase of Desulfovibrio gigas on amino-terminated alkanethiol self-assembled monolayers (SAMs) with different levels of protonation. Classical all-atom molecular dynamics (MD) simulations revealed a strong correlation between the adsorption behavior and the level of ionization of the chemically modified electrode surface. While the hydrogenase undergoes a weak but stable initial adsorption process on SAMs with a low degree of protonation, a stronger immobilization is observable on highly ionized SAMs, affecting protein reorientation and conformation. These results were validated by complementary surface-enhanced infrared absorption (SEIRA) measurements on the comparable [NiFe] standard hydrogenases from Desulfovibrio vulgaris Miyazaki F and allowed in this way for a detailed insight into the adsorption mechanism at the atomic level.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Chemical Society
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
[Nife] Hydrogenase
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Self-Assembled Monolayers
dc.subject.classification
Físico-Química, Ciencia de los Polímeros, Electroquímica
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dc.subject.classification
Ciencias Químicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Effect of the Protonation Degree of a Self-Assembled Monolayer on the Immobilization Dynamics of a [NiFe] Hydrogenase
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-10-25T19:28:29Z
dc.journal.volume
29
dc.journal.number
2
dc.journal.pagination
673-682
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Washington
dc.description.fil
Fil: Utesch, Tillmann. Technishe Universitat Berlin; Alemania
dc.description.fil
Fil: Millo, Diego. Vrije Universiteit Amsterdam; Países Bajos. Technishe Universitat Berlin; Alemania
dc.description.fil
Fil: Castro, Maria Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de Los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires; Argentina
dc.description.fil
Fil: Hildebrandt, Peter . Technishe Universitat Berlin; Alemania
dc.description.fil
Fil: Zebger, Ingo . Technishe Universitat Berlin; Alemania
dc.description.fil
Fil: Mroginski, Maria Andrea . Technishe Universitat Berlin; Alemania
dc.journal.title
Langmuir
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/abs/10.1021/la303635q
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1021/la303635q
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