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dc.contributor.author
Güerci, Alba Mabel
dc.contributor.author
Liviac, D.
dc.contributor.author
Marcos, R.
dc.date.available
2019-07-15T23:08:28Z
dc.date.issued
2009-02
dc.identifier.citation
Güerci, Alba Mabel; Liviac, D.; Marcos, R.; Detection of excision repaired DNA damage in the comet assay by using Ara-C and hydroxyurea in three different cell types; Springer; Cell Biology And Toxicology; 25; 1; 2-2009; 73-80
dc.identifier.issn
0742-2091
dc.identifier.uri
http://hdl.handle.net/11336/79609
dc.description.abstract
Because of its characteristics, the comet assay has been used to evaluate the ability of virtually any type of eukaryotic cell to repair different kinds of DNA damage, including double and single strand breaks and base damage. The ability to detect excision repair sites using the alkaline version can be enhanced by the inclusion of repair inhibitors, DNA synthesis inhibitors, or chain terminators. In this sense, we evaluated the ability of hydroxyurea (HU) and cytosine arabinoside (Ara-C), for detecting lesions produced by the alkylating agents ethyl methanesulfonate (EMS) and methyl methanesulfonate (MMS) in three different cell systems. Two hundred cells for experimental point were analyzed in the alkaline version of the comet assay, and the results are evidences of the utility of the assay to detect alkylation of bases in the cells lines MRC-5 and TK-6, as the treatment with HU +Ara-C significantly increases both the basal and induced frequency of DNA damage. The use of whole blood, although it detected the effects of MMS, with and without repair inhibitors, failed to detect the effect of the selected dose of EMS and does not permit detection increases in the background level.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Alkylating Agents
dc.subject
Ara-C
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Comet Assay
dc.subject
Human Cell Lines
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Hydroxyurea
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Genética Humana
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Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Detection of excision repaired DNA damage in the comet assay by using Ara-C and hydroxyurea in three different cell types
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-07-02T16:24:00Z
dc.journal.volume
25
dc.journal.number
1
dc.journal.pagination
73-80
dc.journal.pais
Alemania
dc.journal.ciudad
Berlín
dc.description.fil
Fil: Güerci, Alba Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Universitat Autònoma de Barcelona; España
dc.description.fil
Fil: Liviac, D.. Universitat Autònoma de Barcelona; España
dc.description.fil
Fil: Marcos, R.. Universitat Autònoma de Barcelona; España. Universidad Carlos III de Madrid. Instituto de Salud; España
dc.journal.title
Cell Biology And Toxicology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s10565-007-9042-x
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10565-007-9042-x
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