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dc.contributor.author
Vazquez, Martin Pablo
dc.contributor.author
Mualem, David
dc.contributor.author
Bercovich, Natalia
dc.contributor.author
Stern, Michael Zeev
dc.contributor.author
Nyambega, Benson
dc.contributor.author
Barda, Omer
dc.contributor.author
Masiga, Dan
dc.contributor.author
Gupta, Sachin Kumar
dc.contributor.author
Michaeli, Shulamit
dc.contributor.author
Levin, Mariano Jorge
dc.date.available
2019-07-12T17:49:48Z
dc.date.issued
2009-04
dc.identifier.citation
Vazquez, Martin Pablo; Mualem, David; Bercovich, Natalia; Stern, Michael Zeev; Nyambega, Benson; et al.; Functional characterization and protein-protein interactions of trypanosome splicing factors U2AF35, U2AF65 and SF1; Elsevier Science; Molecular and Biochemical Parasitology; 164; 2; 4-2009; 137-146
dc.identifier.issn
0166-6851
dc.identifier.uri
http://hdl.handle.net/11336/79490
dc.description.abstract
Early in the assembly of the spliceosome of eukaryotes the branch-point binding protein (BBP, also called SF1) recognizes the branch point sequence, whereas the heterodimer U2AF consisting of a 65 and a 35 kDa subunit, contacts the polypyrimidine tract and the AG splice site, respectively. Herein, we identified, cloned and expressed the Trypanosoma cruzi and Trypanosoma brucei U2AF35, U2AF65 and SF1. Trypanosomatid U2AF65 strongly diverged from yeast and human homologues. On the contrary, trypanosomatid SF1 was conserved but lacked the C-terminal sequence present in the mammalian protein. Yeast two hybrid approaches were used to assess their interactions. The interaction between U2AF35 and U2AF65 was very weak or not detectable. However, as in other eukaryotes, the interaction between U2AF65 and SF1 was strong. At the cellular level, these results were confirmed by fractionation and affinity-selection experiments in which SF1 and U2AF65 co-fractionated in a complex of approximately 400 kDa and U2AF65 was affinity-selected with TAP tagged SF1, but not with TAP tagged U2AF35. Silencing of the three factors affected growth and trans-splicing in the first step of this reaction. Trypanosomes are the first described example of eukaryotic cells in which the interaction of two expressed U2AF factors seemed to be very weak, or even undetectable.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Trans-Splicing
dc.subject
Trypanosoma Brucei
dc.subject
Trypanosoma Cruzi
dc.subject
U2 Auxiliary Factor
dc.subject.classification
Biología Celular, Microbiología
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Functional characterization and protein-protein interactions of trypanosome splicing factors U2AF35, U2AF65 and SF1
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-07-11T19:23:22Z
dc.journal.volume
164
dc.journal.number
2
dc.journal.pagination
137-146
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Vazquez, Martin Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
dc.description.fil
Fil: Mualem, David. Bar-Ilan University; Israel
dc.description.fil
Fil: Bercovich, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
dc.description.fil
Fil: Stern, Michael Zeev. Bar-Ilan University; Israel
dc.description.fil
Fil: Nyambega, Benson. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. International Center for Insect Physiology and Ecology; Kenia
dc.description.fil
Fil: Barda, Omer. Bar-Ilan University; Israel
dc.description.fil
Fil: Masiga, Dan. International Center for Insect Physiology and Ecology; Kenia
dc.description.fil
Fil: Gupta, Sachin Kumar. Bar-Ilan University; Israel
dc.description.fil
Fil: Michaeli, Shulamit. Bar-Ilan University; Israel
dc.description.fil
Fil: Levin, Mariano Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Inserm; Francia
dc.journal.title
Molecular and Biochemical Parasitology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.molbiopara.2008.12.009
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0166685109000024
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