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dc.contributor.author
Bercovich, Natalia
dc.contributor.author
Levin, Mariano Jorge
dc.contributor.author
Vazquez, Martin Pablo
dc.date.available
2019-07-12T17:45:02Z
dc.date.issued
2009-03
dc.identifier.citation
Bercovich, Natalia; Levin, Mariano Jorge; Vazquez, Martin Pablo; The FIP-1 like polyadenylation factor in trypanosomes and the structural basis for its interaction with CPSF30; Academic Press Inc Elsevier Science; Biochemical and Biophysical Research Communications; 380; 4; 3-2009; 850-855
dc.identifier.issn
0006-291X
dc.identifier.uri
http://hdl.handle.net/11336/79488
dc.description.abstract
In trypanosomes transcription is polycistronic and individual mRNAs are generated by a trans-splicing/polyadenylation coupled reaction. We identified a divergent trypanosome FIP1-like, a factor required for mRNA 3′ end formation from yeasts to human. Here we showed that it is a nuclear protein with a speckled distribution essential for trypanosome viability. A strong interaction was found between TcFIP1-like and TcCPSF30, a component of the polyadenylation complex. We determined the specific amino acids in each protein involved in the interaction. Significant differences were found between the trypanosome interaction surface and its human counterpart. Although CPSF30/FIP1 interaction is known in other organisms, this is the first report mapping the interaction surface at the amino acid level.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Academic Press Inc Elsevier Science
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Drug Target
dc.subject
Mrna Processing
dc.subject
Polyadenylation Factors
dc.subject
Protein Interactions
dc.subject
Trypanosome Diseases
dc.subject.classification
Biología Celular, Microbiología
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
The FIP-1 like polyadenylation factor in trypanosomes and the structural basis for its interaction with CPSF30
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-07-11T19:23:26Z
dc.journal.volume
380
dc.journal.number
4
dc.journal.pagination
850-855
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Bercovich, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
dc.description.fil
Fil: Levin, Mariano Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
dc.description.fil
Fil: Vazquez, Martin Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
dc.journal.title
Biochemical and Biophysical Research Communications
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/19338765
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://doi.org/10.1016/j.bbrc.2009.01.182


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