Artículo
Culturing bacteria from caenorhabditis elegans gut to assess colonization proficiency
Rodriguez Ayala, Facundo; Cogliati, Sebastian Claudio
; Bauman, Carlos; Leñini, Cecilia Andrea
; Bartolini, Marco
; Villalba, Juan Manuel; Argañaraz, Federico; Grau, Roberto Ricardo
Fecha de publicación:
06/2017
Editorial:
Bio-Protocol
Revista:
Bio-Protocol
ISSN:
2331-8325
e-ISSN:
2331-8325
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Determining an accurate count of intestinal bacteria from Caenorhabditis elegans is onecritical way to assess colonization proficiency by a given bacteria. This can be accomplished by culturingappropriate dilutions of worm gut bacteria on selective or differential agarized media. Because of thehigh concentration of bacteria in gut worm, dilution is necessary before plating onto growth media.Serial dilutions can reduce the concentration of the original intestinal sample to levels low enough forsingle colonies to be grown on media plates, allowing for the calculation of the initial counts of bacteriain the intestinal sample.
Palabras clave:
Caenorhabditis Elegans
,
Bacillus Subtilis
,
Escherischia Coli
,
Culture
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CCT - ROSARIO)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - ROSARIO
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - ROSARIO
Citación
Rodriguez Ayala, Facundo; Cogliati, Sebastian Claudio; Bauman, Carlos; Leñini, Cecilia Andrea; Bartolini, Marco; et al.; Culturing bacteria from caenorhabditis elegans gut to assess colonization proficiency; Bio-Protocol; Bio-Protocol; 7; 12; 6-2017; 1-9
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