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dc.contributor.author
Martinez, Maria Alejandra
dc.contributor.author
Delgado, Osvaldo Daniel
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Breccia, Javier Dario
dc.contributor.author
Baigori, Mario Domingo
dc.contributor.author
Siñeriz, Faustino
dc.date.available
2019-07-05T13:55:56Z
dc.date.issued
2002-06
dc.identifier.citation
Martinez, Maria Alejandra; Delgado, Osvaldo Daniel; Breccia, Javier Dario; Baigori, Mario Domingo; Siñeriz, Faustino; Revision of the taxonomic position of the xylanolytic Bacillus sp. MIR32 reidentified as Bacillus halodurans and plasmid-mediated transformation of B. halodurans; Springer Tokyo; Extremophiles; 6; 5; 6-2002; 391-395
dc.identifier.issn
1431-0651
dc.identifier.uri
http://hdl.handle.net/11336/79206
dc.description.abstract
Bacillus sp. MIR32 has been isolated using xylan as the only carbon source, and one of its xylanolytic enzymes has been extensively studied. Biochemical analysis first related this strain to Bacillus amyloliquefaciens, but further studies based on a comparison of 16S rDNA sequences, G+C content, and DNA–DNA hybridization showed that strain MIR32 should be classified as a member of the species Bacillus halodurans. This change is also supported by the typical phenotype observed and by the results of PCR amplification directed toward spacers in rDNA and tDNA genes, which were assayed and compared with those of B. halodurans DSM 497T. Although among alkaliphilic bacilli competence development has not been experimentally demonstrated, in this work both B. halodurans MIR32 and DSM 497T were transformed according to a simple procedure developed in our laboratory, reaching 102–103 stable transformants per microgram of plasmid DNA.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer Tokyo
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
16s Rdna Sequence Analysis
dc.subject
16s-23s Intergenic Spacer Region
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B. Halodurans Mir32
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Dna-Dna Hybridization
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Molecular Characterization
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Transformation
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Biología Celular, Microbiología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
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Bioquímica y Biología Molecular
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Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Revision of the taxonomic position of the xylanolytic Bacillus sp. MIR32 reidentified as Bacillus halodurans and plasmid-mediated transformation of B. halodurans
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-06-14T15:19:40Z
dc.journal.volume
6
dc.journal.number
5
dc.journal.pagination
391-395
dc.journal.pais
Japón
dc.journal.ciudad
Tokyo
dc.description.fil
Fil: Martinez, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Delgado, Osvaldo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Breccia, Javier Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Baigori, Mario Domingo. Universidad Nacional de Tucumán; Argentina
dc.description.fil
Fil: Siñeriz, Faustino. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.journal.title
Extremophiles
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.springerlink.com/content/bljcpjae9cactrr5/?p=753b3ed13a864e3d80dd0babcc5718ad&pi=5
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1007/s00792-002-0269-4
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