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dc.contributor.author Martinez, Maria Alejandra
dc.contributor.author Delgado, Osvaldo Daniel
dc.contributor.author Breccia, Javier Dario
dc.contributor.author Baigori, Mario Domingo
dc.contributor.author Siñeriz, Faustino
dc.date.available 2019-07-05T13:55:56Z
dc.date.issued 2002-06
dc.identifier.citation Martinez, Maria Alejandra; Delgado, Osvaldo Daniel; Breccia, Javier Dario; Baigori, Mario Domingo; Siñeriz, Faustino; Revision of the taxonomic position of the xylanolytic Bacillus sp. MIR32 reidentified as Bacillus halodurans and plasmid-mediated transformation of B. halodurans; Springer Tokyo; Extremophiles; 6; 5; 6-2002; 391-395
dc.identifier.issn 1431-0651
dc.identifier.uri http://hdl.handle.net/11336/79206
dc.description.abstract Bacillus sp. MIR32 has been isolated using xylan as the only carbon source, and one of its xylanolytic enzymes has been extensively studied. Biochemical analysis first related this strain to Bacillus amyloliquefaciens, but further studies based on a comparison of 16S rDNA sequences, G+C content, and DNA–DNA hybridization showed that strain MIR32 should be classified as a member of the species Bacillus halodurans. This change is also supported by the typical phenotype observed and by the results of PCR amplification directed toward spacers in rDNA and tDNA genes, which were assayed and compared with those of B. halodurans DSM 497T. Although among alkaliphilic bacilli competence development has not been experimentally demonstrated, in this work both B. halodurans MIR32 and DSM 497T were transformed according to a simple procedure developed in our laboratory, reaching 102–103 stable transformants per microgram of plasmid DNA.
dc.format application/pdf
dc.language.iso eng
dc.publisher Springer Tokyo
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject 16S RDNA SEQUENCE ANALYSIS
dc.subject 16S-23S INTERGENIC SPACER REGION
dc.subject B. HALODURANS MIR32
dc.subject DNA-DNA HYBRIDIZATION
dc.subject MOLECULAR CHARACTERIZATION
dc.subject TRANSFORMATION
dc.subject.classification Biología Celular, Microbiología
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.subject.classification Bioquímica y Biología Molecular
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Revision of the taxonomic position of the xylanolytic Bacillus sp. MIR32 reidentified as Bacillus halodurans and plasmid-mediated transformation of B. halodurans
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2019-06-14T15:19:40Z
dc.journal.volume 6
dc.journal.number 5
dc.journal.pagination 391-395
dc.journal.pais Japón
dc.journal.ciudad Tokyo
dc.description.fil Fil: Martinez, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil Fil: Delgado, Osvaldo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil Fil: Breccia, Javier Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil Fil: Baigori, Mario Domingo. Universidad Nacional de Tucumán; Argentina
dc.description.fil Fil: Siñeriz, Faustino. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.journal.title Extremophiles
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://www.springerlink.com/content/bljcpjae9cactrr5/?p=753b3ed13a864e3d80dd0babcc5718ad&pi=5
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1007/s00792-002-0269-4
dc.conicet.fuente individual


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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)