Artículo
Paneth cell identification in the small intestine of guinea pig offsprings (Cavia porcellus)
Vásquez Cachay, María Elith; Pebe, Erika; Rodríguez Gutiérrez, José Luis; Mejía, Boris Lira; Falcón Pérez, Néstor; Zanuzzi, Carolina Natalia
; Barbeito, Claudio Gustavo


Fecha de publicación:
01/2014
Editorial:
Wiley-liss, Div John Wiley & Sons Inc
Revista:
Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology
ISSN:
1932-8486
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The aim of this study was to determine the presence, number, and morphometrical characteristics of Paneth cells (PC) in the small intestine of guinea pigs during lactation. We used 48 pups from 0 to 15 days old. Samples from small intestine were fixed in 10% buffered formaldehyde (pH 7.4) and processed for histological and morphometrical studies using hematoxylin and eosin (HE), Phloxine tartrazine or Masson's Trichome staining, or immunohistochemistry for lysozyme. PC were morphologically identified at day 2 using Masson's Trichome or Phloxine tartrazine stainings, and at day 4 using HE, whereas using immunohistochemistry they were recognized from birth. Morphometrical differences were found between the intestinal sections at each age studied, and within each section during the first weeks of life. In all developmental stage, the highest number of PC was observed in the duodenum of 13 days old guinea pigs. Our results confirm the presence of PC in the small intestine of guinea pigs from birth.
Palabras clave:
Guinea Pig
,
Immunohistochemistry
,
Lactation
,
Lysozyme
,
Paneth Cells
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Articulos(CCT - LA PLATA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Citación
Vásquez Cachay, María Elith; Pebe, Erika; Rodríguez Gutiérrez, José Luis; Mejía, Boris Lira; Falcón Pérez, Néstor; et al.; Paneth cell identification in the small intestine of guinea pig offsprings (Cavia porcellus); Wiley-liss, Div John Wiley & Sons Inc; Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology; 297; 5; 1-2014; 856-863
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