Mostrar el registro sencillo del ítem
dc.contributor.author
Villegas Gabutti, Carlos Mauricio
dc.contributor.author
Ezquer, Eduardo Marcelo
dc.contributor.author
Deis, Ricardo
dc.contributor.author
Maldonado, Cristina Alicia
dc.contributor.author
Soaje, Marta
dc.date.available
2019-07-04T17:37:13Z
dc.date.issued
2009-10
dc.identifier.citation
Villegas Gabutti, Carlos Mauricio; Ezquer, Eduardo Marcelo; Deis, Ricardo; Maldonado, Cristina Alicia; Soaje, Marta; Pituitary changes involved in prolactin secretion induced by mifepristone and naloxone during late pregnancy; Karger; Neuroendocrinology; 89; 2; 10-2009; 200-209
dc.identifier.issn
0028-3835
dc.identifier.uri
http://hdl.handle.net/11336/79143
dc.description.abstract
The antiprogesterone mifepristone facilitates prolactin release, an effect enhanced by administration of the opioid antagonist naloxone. The present study explores ultrastructural changes in lactotropes after mifepristone and naloxone administration, correlating them with the expression of pituitary prolactin. Methods/Results: Rats were sacrificed at 18:00 h on day 19 of pregnancy. Prolactin immunoelectron microscopy of lactotropes from control rats showed characteristics of quiescent cells with numerous small and spherical secretory granules. Naloxone administration did not modify lactotrope morphology or prolactin expression in terms of mRNA or protein abundances. Mifepristone treatment induced lactotrope activation with development of the rough endoplasmic reticulum and Golgi complex with prolactin immunoreactive small newly formed and large mature secretory granules. Mifepristone increased prolactin mRNA and protein expression. Naloxone administration to mifepristone-treated rats potentiated lactotrope activation compared with mifepristone alone showing exocytotic images of prolactin granules and some cells with evident signs of involution. Conclusions: (1) Blockade of progesterone action by mifepristone activated the lactotrope, increased significantly prolactin mRNA and protein expression and prepared the pituitary for naloxone action. (2) The high serum prolactin levels induced by mifepristone and naloxone may regulate negatively lactotrope activity as suggested by the presence of regressing cells neighboring the actively secreting cells.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Karger
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Mifepristone
dc.subject
Naloxone
dc.subject
Opiate Peptides
dc.subject
Pituitary
dc.subject
Pregnancy
dc.subject
Progesterone
dc.subject
Prolactin
dc.subject.classification
Otras Ciencias de la Salud
dc.subject.classification
Ciencias de la Salud
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Pituitary changes involved in prolactin secretion induced by mifepristone and naloxone during late pregnancy
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-06-10T21:41:55Z
dc.identifier.eissn
1423-0194
dc.journal.volume
89
dc.journal.number
2
dc.journal.pagination
200-209
dc.journal.pais
Suiza
dc.journal.ciudad
Basel
dc.description.fil
Fil: Villegas Gabutti, Carlos Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
dc.description.fil
Fil: Ezquer, Eduardo Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
dc.description.fil
Fil: Deis, Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
dc.description.fil
Fil: Maldonado, Cristina Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
dc.description.fil
Fil: Soaje, Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
dc.journal.title
Neuroendocrinology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.karger.com/Article/Abstract/161113
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1159/000161113
Archivos asociados