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dc.contributor.author
Rubio, María Cristina
dc.contributor.author
Runco, Rosa
dc.contributor.author
Navarro, Antonio Roberto
dc.date.available
2019-07-04T14:21:39Z
dc.date.issued
2002-11
dc.identifier.citation
Rubio, María Cristina; Runco, Rosa; Navarro, Antonio Roberto; Invertase from a strain of Rhodotorula glutinis; Pergamon-Elsevier Science Ltd; Phytochemistry; 61; 6; 11-2002; 605-609
dc.identifier.issn
0031-9422
dc.identifier.uri
http://hdl.handle.net/11336/79116
dc.description.abstract
An invertase (β-D-fructofuranoside fructohydrolase, EC 3.2.1.26) from Rhodotorula glutinis was purified by ammonium sulfate fractionation, gel filtration and anion exchange chromatography. Invertase molecular weight was estimated to be 100 kDa by analytical gel filtration and 47 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Molecular mass determinations indicated that the native enzyme exists as a homodimer. It is a glycoprotein that contains 19% carbohydrate. The enzyme attacks β-D-fructofuranoside (raffinose, stachyose and sucrose) from the fructose end. It has a Km of 0.227 M and a Vmax of 0.096 μmol/min with sucrose as a substrate. Invertase activity is stable between pH 2.6 and 5.5 for 30 min, maximum activity being observed at pH 4.5. The activation energy was 6520 cal/mol. The enzyme is stable between 20 and 60°C. Mg2+ and Ca2+ ions stimulated invertase activity 3-fold, while Fe2+, K+, Co2+, Na+ and Cu2+ increased activity about 2-fold. The transfructosylation reaction could not be observed. This enzyme is of particular interest since it appears to have a high hydrolytic activity in 1 M sucrose solution. This fact would make the enzymatic hydrolysis process economically efficient for syrup production using by-products with high salt and sugar contents such as sugar cane molasses. © 2002 Elsevier Science Ltd. All rights reserved.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Pergamon-Elsevier Science Ltd
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Β-Fructofuranoside Fructohydrolase
dc.subject
Enzymatic Characterization
dc.subject
Food Industry
dc.subject
Hemiascomycetidae
dc.subject
Invert Sugars
dc.subject
Invertase
dc.subject
Rhodotorula Glutinis
dc.subject.classification
Bioprocesamiento Tecnológico, Biocatálisis, Fermentación
dc.subject.classification
Biotecnología Industrial
dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
dc.title
Invertase from a strain of Rhodotorula glutinis
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-06-14T15:17:15Z
dc.journal.volume
61
dc.journal.number
6
dc.journal.pagination
605-609
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Nueva York
dc.description.fil
Fil: Rubio, María Cristina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
dc.description.fil
Fil: Runco, Rosa. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
dc.description.fil
Fil: Navarro, Antonio Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
dc.journal.title
Phytochemistry
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/S0031-9422(02)00336-9
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0031942202003369
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