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dc.contributor.author
Martínez Rincón, Raúl O.
dc.contributor.author
Rivera Pérez, Crisalejandra
dc.contributor.author
Diambra, Luis Anibal
dc.contributor.author
Noriega, Fernando Gabriel
dc.date.available
2019-07-01T19:53:38Z
dc.date.issued
2017-02-03
dc.identifier.citation
Martínez Rincón, Raúl O.; Rivera Pérez, Crisalejandra; Diambra, Luis Anibal; Noriega, Fernando Gabriel; Modeling the flux of metabolites in the juvenile hormone biosynthesis pathway using generalized additive models and ordinary differential equations; Public Library of Science; Plos One; 12; 2; 3-2-2017; 1-21
dc.identifier.issn
1932-6203
dc.identifier.uri
http://hdl.handle.net/11336/78966
dc.description.abstract
Juvenile hormone (JH) regulates development and reproductive maturation in insects. Thecorpora allata (CA) from female adult mosquitoes synthesize fluctuating levels of JH, whichhave been linked to the ovarian development and are influenced by nutritional signals. Therate of JH biosynthesis is controlled by the rate of flux of isoprenoids in the pathway, whichis the outcome of a complex interplay of changes in precursor pools and enzyme levels. Acomprehensive study of the changes in enzymatic activities and precursor pool sizes havebeen previously reported for the mosquito Aedes aegypti JH biosynthesis pathway. In thepresent studies, we used two different quantitative approaches to describe and predict howchanges in the individual metabolic reactions in the pathway affect JH synthesis. First, weconstructed generalized additive models (GAMs) that described the association betweenchanges in specific metabolite concentrations with changes in enzymatic activities and sub-strate concentrations. Changes in substrate concentrations explained 50% or more of themodel deviances in 7 of the 13 metabolic steps analyzed. Addition of information on enzy-matic activities almost always improved the fitness of GAMs built solely based on substrateconcentrations. GAMs were validated using experimental data that were not included whenthe model was built. In addition, a system of ordinary differential equations (ODE) wasdeveloped to describe the instantaneous changes in metabolites as a function of the levelsof enzymatic catalytic activities. The results demonstrated the ability of the models to predictchanges in the flux of metabolites in the JH pathway, and can be used in the future to designand validate experimental manipulations of JH synthesis.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Public Library of Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
Juvenile Hormone Synthesis
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Modeling Biosynthesis
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Aedes Aegypti
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Mevalonic Acid Pathway
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Otras Ciencias Biológicas
dc.subject.classification
Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Modeling the flux of metabolites in the juvenile hormone biosynthesis pathway using generalized additive models and ordinary differential equations
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-05-23T19:12:23Z
dc.journal.volume
12
dc.journal.number
2
dc.journal.pagination
1-21
dc.journal.pais
Estados Unidos
dc.journal.ciudad
San Francisco
dc.description.fil
Fil: Martínez Rincón, Raúl O.. Consejo Nacional de Ciencia y Tecnología; México. Centro de Investigaciones Biológicas del Noroeste; México
dc.description.fil
Fil: Rivera Pérez, Crisalejandra. Consejo Nacional de Ciencia y Tecnología; México. Centro de Investigaciones Biológicas del Noroeste; México
dc.description.fil
Fil: Diambra, Luis Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina
dc.description.fil
Fil: Noriega, Fernando Gabriel. Florida International University; Estados Unidos
dc.journal.title
Plos One
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/ 10.1371/journal.pone.0171516
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0171516
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