Artículo
Construction and Validation of a Dose-Response Curve Using the Comet Assay to Determine Human Radiosensitivity to Ionizing Radiation
Fecha de publicación:
27/08/2011
Editorial:
Taylor & Francis
Revista:
Journal of Toxicology and Environmental Health-Part A-Current Issues
ISSN:
1528-7394
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Individual radiosensitivity is an individual characteristic associated with an increased reaction to ionizing radiation. The purpose of our work is to establish a dose-response curve useful to classify individuals as radiosensitive or radioresistant. Thus, a dose-response curve was constructed by measuring in vitro responses to increasing doses (0 to 8 Gy) of gamma radiation in the comet assay. The obtained curve fit well with a linear equation in the range of 0 to 8 Gy. The overall dose-response curve was constructed for percent DNA in tail, as a measure of the genetic damage induced by irradiation. To probe the goodness of the constructed curve, a validation study was carried out with whole blood from two donors in a blind study. Results show that, for the two applied doses (2 and 6 Gy), the obtained values fit well inside the interval of confidence of the curve. In conclusion, our results demonstrate the usefulness of the comet assay in determining individual responses to defined doses of gamma radiation. The standard dose-response curve constructed may be used to detect individuals departing from reference values.
Palabras clave:
Radiosensitivity
,
Ionizing Radiation
,
Comet Assay
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Articulos(IGEVET)
Articulos de INST.DE GENETICA VET ING FERNANDO NOEL DULOUT
Articulos de INST.DE GENETICA VET ING FERNANDO NOEL DULOUT
Citación
Güerci, Alba Mabel; Zúñiga, L.; Marcos, R.; Construction and Validation of a Dose-Response Curve Using the Comet Assay to Determine Human Radiosensitivity to Ionizing Radiation
; Taylor & Francis; Journal of Toxicology and Environmental Health-Part A-Current Issues; 74; 15-16; 27-8-2011; 1087-1093
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