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dc.contributor.author
Schell, Christoph
dc.contributor.author
Albrecht, Martín
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dc.contributor.author
Mayer, Christine
dc.contributor.author
Ullrich Schwarzer, J.
dc.contributor.author
Frungieri, Monica Beatriz
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dc.contributor.author
Mayerhofer, Artur
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dc.date.available
2019-06-24T14:55:42Z
dc.date.issued
2008-04
dc.identifier.citation
Schell, Christoph; Albrecht, Martín; Mayer, Christine; Ullrich Schwarzer, J.; Frungieri, Monica Beatriz; et al.; Exploring human testicular peritubular cells: Identification of secretory products and regulation by tumor necrosis factor-α; Endocrine Society; Endocrinology; 149; 4; 4-2008; 1678-1686
dc.identifier.issn
0013-7227
dc.identifier.uri
http://hdl.handle.net/11336/78706
dc.description.abstract
Testicular peritubular cells are myofibroblastic cells, which represent the major cellular components of the wall of the seminiferous tubules. In men their phenotypic characteristics, including possible secretory activity and regulation, are not well known, in neither normal nor pathologically altered testes. Especially in testes of men with impaired spermatogenesis, the cytoarchitecture of the tubular wall is frequently remodeled and presents fibrotic thickening, increased innervation, and infiltration by macrophages and mast cells. The latter are two sources of TNF-α. The purpose of our study was to explore human testicular peritubular cells and mechanisms of their regulation. To this end we primarily studied cultured human testicular peritubular cells (HTPCs), isolated from adult human testes. Having established that HTPCs express TNF-α receptors 1 and 2 and respond to recombinant human TNF-α by a rapid phosphorylation of ERK1/2, we used complementary approaches, including gene array/RT-PCR studies, Western blotting/ immunocytochemistry, and ELISA techniques to study phenotypic characteristics of HTPCs and actions of TNFα. We found that HTPCs express the nerve growth factor gene and TNF-α-stimulated mRNA levels and secretion of nerve growth factor in a dose- and time-dependent manner. Similarly, monocyte chemoattractant protein-1 was identified as a product of HTPCs, which was regulated by TNF-α in a concentration- and time-dependent manner. TNF-α furthermore strongly enhanced expression and/or synthesis of other inflammatory molecules, namely IL-6 and cyclooxygenase-2. Active cyclooxygenase-2 is indicated by increased prostaglandin D2 levels. In addition, intercellular adhesion molecule-1, which was not detected at protein level in the absence of TNF-α, was induced upon TNF-α stimulation. In conclusion, these results provide novel insights into the nature of human peritubular cells, which are able to secrete potent signaling molecules and are regulated by TNF-α. These results also hint to an as-yet-unknown role of peritubular cells in normal human testis and involvement in the pathomechanisms associated with impaired spermatogenesis in men. Copyright © 2008 by The Endocrine Society.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Endocrine Society
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Testis
dc.subject
Peritubular Cells
dc.subject
Tnfalpha
dc.subject
Human
dc.subject.classification
Inmunología
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dc.subject.classification
Medicina Básica
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dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
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dc.subject.classification
Biología Reproductiva
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Exploring human testicular peritubular cells: Identification of secretory products and regulation by tumor necrosis factor-α
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-05-14T15:51:55Z
dc.journal.volume
149
dc.journal.number
4
dc.journal.pagination
1678-1686
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Chevy Chase
dc.description.fil
Fil: Schell, Christoph. Ludwig Maximilians Universitat; Alemania
dc.description.fil
Fil: Albrecht, Martín. Ludwig Maximilians Universitat; Alemania
dc.description.fil
Fil: Mayer, Christine. Ludwig Maximilians Universitat; Alemania
dc.description.fil
Fil: Ullrich Schwarzer, J.. Academic Teaching Hospital Freising; Alemania
dc.description.fil
Fil: Frungieri, Monica Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil
Fil: Mayerhofer, Artur. Ludwig Maximilians Universitat; Alemania
dc.journal.title
Endocrinology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/endo/article/149/4/1678/2455141
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1210/en.2007-1064
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