Artículo
Papaya ringspot virus W infecting Luffa aegyptiaca in Cuba
Cabrera Mederos, Dariel
; Giolitti, Fabián José; Nome, Claudia; Bejerman, Nicolas
; Portal Villafaña, Orelvis
![Icon](/themes/CONICETDigital/images/conicet.png)
![Icon](/themes/CONICETDigital/images/conicet.png)
Fecha de publicación:
06/2017
Editorial:
Springer Netherlands
Revista:
Australasian Plant Disease Notes
e-ISSN:
1833-928X
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Luffa aegyptiaca (sponge gourd) plants showing severe leaf mosaic and deformation symptoms were observed in Villa Clara, Cuba. Electron microscopy observations of leaf dip preparations revealed flexuous filamentous particles, which were identified as Papaya ringspot virus (PRSV) by ELISA test. PRSV was mechanically transmitted to healthy Cucurbita moschata plants. Sequence analysis of the RT-PCR product obtained from the capsid protein gene showed highest identity with other PRSV isolates from United States of America, Australia, Venezuela, Brazil, India and Cuba, ranging between 93.2–96.5% and 93.4–98.1% for nucleotide and amino acid sequences, respectively. Phylogenetic analysis showed two main clusters. Cluster I included isolates from the Americas-Australia group and India, including sponge gourd isolate, while the cluster II included isolates from China, Thailand and Taiwan. To our knowledge, this is the first report of PRSV-W infecting sponge gourd in Cuba.
Palabras clave:
Papaya Ringspot Virus
,
Sequencing
,
Sponge Gourd
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CCT - CORDOBA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA
Citación
Cabrera Mederos, Dariel; Giolitti, Fabián José; Nome, Claudia; Bejerman, Nicolas; Portal Villafaña, Orelvis; Papaya ringspot virus W infecting Luffa aegyptiaca in Cuba; Springer Netherlands; Australasian Plant Disease Notes; 12; 1; 6-2017
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