Mostrar el registro sencillo del ítem

dc.contributor.author
Perotti, Valeria Elisa
dc.contributor.author
Moreno, Alejandra S.
dc.contributor.author
Tripodi, Karina Eva Josefina
dc.contributor.author
Meier, Guillermo
dc.contributor.author
Bello, Fernando
dc.contributor.author
Cocco, Mariángeles
dc.contributor.author
Vázquez, Daniel
dc.contributor.author
Anderson, Catalina
dc.contributor.author
Podesta, Florencio Esteban
dc.date.available
2016-10-28T21:22:47Z
dc.date.issued
2014-05
dc.identifier.citation
Perotti, Valeria Elisa; Moreno, Alejandra S.; Tripodi, Karina Eva Josefina; Meier, Guillermo; Bello, Fernando; et al.; Proteomic and metabolomic profiling of Valencia orange fruit after natural frost exposure; Wiley; Physiologia Plantarum; 153; 3; 5-2014; 337–354
dc.identifier.issn
0031-9317
dc.identifier.uri
http://hdl.handle.net/11336/7861
dc.description.abstract
The aim of this study was to evaluate the response of orange fruit (Citrus sinensis var. Valencia Late) to freezing stress in planta, both immediately after the natural event and after a week, in order to understand the biochemical and molecular basis of the changes that later derive in internal and external damage symptoms. Using two-dimensional differential gel electrophoresis to analyze exposed and non-exposed fruit, 27 differential protein spots were detected in juice sacs and flavedo, among all comparisons made. Also, primary and secondary metabolites relative contents were analyzed in both tissues by gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry, respectively. Proteins and compounds involved in regulatory functions, iron metabolism, oxidative damage and carbohydrate metabolism were the most affected. Interestingly, three glycolytic enzymes were induced by cold, and there was an increase in fermentation products (volatiles); all of that suggests that more energy generation might be required from glycolysis to counter the cold stress. Moreover, a notable increase in sugar levels was observed after frost, but it was not at the expense of organic acids utilization. Consequently, these results suggest a probable redistribution of photoassimilates in the frost-exposed plants, tending to restore the homeostasis altered by that severe type of stress. Isosinensetin was the most cold-sensitive secondary metabolite because it could not be detected at all after the frost, constituting a possible tool to early diagnose freezing damage.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Citrus
dc.subject
Proteomics
dc.subject
Glycolysis
dc.subject
Metabolomics
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Proteomic and metabolomic profiling of Valencia orange fruit after natural frost exposure
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-14T12:50:13Z
dc.journal.volume
153
dc.journal.number
3
dc.journal.pagination
337–354
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Hoboken
dc.description.fil
Fil: Perotti, Valeria Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
dc.description.fil
Fil: Moreno, Alejandra S.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
dc.description.fil
Fil: Tripodi, Karina Eva Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
dc.description.fil
Fil: Meier, Guillermo. Estacion Extal. Agrop. Concordia; Argentina
dc.description.fil
Fil: Bello, Fernando. Estacion Extal. Agrop. Concordia; Argentina
dc.description.fil
Fil: Cocco, Mariángeles. Estacion Extal. Agrop. Concordia; Argentina
dc.description.fil
Fil: Vázquez, Daniel. Estacion Extal. Agrop. Concordia; Argentina
dc.description.fil
Fil: Anderson, Catalina. Estacion Extal. Agrop. Concordia; Argentina
dc.description.fil
Fil: Podesta, Florencio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
dc.journal.title
Physiologia Plantarum
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/ppl.12259/abstract
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/ppl.12259