Artículo
His-containing plant metallothioneins: comparative study of divalent metal-ion binding by plant MT3 and MT4 isoforms
Tomas, Mireia; Pagani, María Ayelén
; Andreo, Carlos Santiago
; Capdevila, Mercè; Bofill, Roger; Atrian, Silvia
![Icon](/themes/CONICETDigital/images/conicet.png)
![Icon](/themes/CONICETDigital/images/conicet.png)
Fecha de publicación:
07/2014
Editorial:
Springer
Revista:
Journal Of Biological Inorganic Chemistry
ISSN:
0949-8257
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Metallothioneins (MTs) are a superfamily of Cys-rich, low-molecular weight metalloproteins that bind heavy metal ions. These cytosolic metallopeptides, which exist in most living organisms, are thought to be involved in metal homeostasis, metal detoxification, and oxidative stress protection. In this work, we characterise the Zn(II)- and Cd(II)-binding abilities of plant type 3 and type 4 MTs identified in soybean and sunflower, both of them being His-containing peptides. The recombinant metal-MT complexes synthesised in Zn(II) or Cd(II)-enriched Escherichia coli cultures have been analysed by ESI-MS, and CD, ICP-AES, and UV spectroscopies. His-to-Ala type 3 MT mutants have also been constructed and synthesised for the study of the role of His in divalent metal ion coordination. The results show comparable divalent metal-binding capacities for the MTs of type 3, and suggest, for the first time, the participation of their conserved C-term His residues in metal binding. Interesting features for the Zn(II)-binding abilities of type 4 MTs are also reported, as their variable His content may be considered crucial for their biological performance.
Palabras clave:
Plant Metallothionein
,
Zinc
,
Cadmium
,
Metal-His-Binding
,
Sulphide Ligands
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CEFOBI)
Articulos de CENTRO DE EST.FOTOSINTETICOS Y BIOQUIMICOS (I)
Articulos de CENTRO DE EST.FOTOSINTETICOS Y BIOQUIMICOS (I)
Citación
Tomas, Mireia; Pagani, María Ayelén; Andreo, Carlos Santiago; Capdevila, Mercè; Bofill, Roger; et al.; His-containing plant metallothioneins: comparative study of divalent metal-ion binding by plant MT3 and MT4 isoforms; Springer; Journal Of Biological Inorganic Chemistry; 19; 7; 7-2014; 1149-1164
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