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Artículo

1α,25(OH)2D3-dependent modulation of Akt in proliferating and differentiating C2C12 skeletal muscle cells

Buitrago, Claudia GracielaIcon ; Arango, Nadia Soledad; Boland, Ricardo LeopoldoIcon
Fecha de publicación: 08/2012
Editorial: Wiley-liss, Div John Wiley & Sons Inc
Revista: Journal of Cellular Biochemistry
ISSN: 0730-2312
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Biológicas

Resumen

We previously reported that 1α,25‐dihydroxy‐vitamin D3 [1α,25(OH)2D3] induces non‐transcriptional rapid responses through activation of Src and MAPKs in the skeletal muscle cell line C2C12. In the present study we investigated the modulation of Akt by the secosteroid hormone in C2C12 cells at proliferative stage (myoblasts) and at early differentiation stage. In proliferating cells, 1α,25(OH)2D3 activates Akt by phosphorylation in Ser473 in a time‐dependent manner (5–60 min). When these cells were pretreated with methyl‐beta‐cyclodextrin to disrupt caveolae microdomains, hormone‐induced activation of Akt was suppressed. Similar results were obtained by siRNA silencing of caveolin‐1 expression, further indicating that hormone effects on cell membrane caveolae are required for downstream signaling. PI3K and p38 MAPK, but not ERK1/2, participate in 1α,25(OH)2D3 activation of Akt in myoblasts. The involvement of p38 MAPK in Akt phosphorylation by the hormone probably occurs through MAPK‐activated protein kinase 2 (MK2), which is activated by the steroid. In addition, the participation of Src in Akt phosphorylation by 1α,25(OH)2D3 was demonstrated using the inhibitor PP2 and antisense oligodeoxynucleotides that suppress Src expression. We also observed that PI3K participates in hormone‐induced proliferation. During the early phase of C2C12 cell differentiation 1α,25(OH)2D3 also increases Akt phosphorylation and activates Src. Of relevance, Src and PI3K are involved in Akt activation and in MHC and myogenin increased expression by 1α,25(OH)2D3. Altogether, these data suggest that 1α,25(OH)2D3 upregulates Akt through Src, PI3K, and p38 MAPK to stimulate myogenesis in C2C12 cells
Palabras clave: 1α,25(Oh)2d3 , Akt And Myogenesis , C2c12 Muscle Cells
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/78333
URL: https://onlinelibrary.wiley.com/doi/abs/10.1002/jcb.23444
DOI: https://doi.org/10.1002/jcb.23444
Colecciones
Articulos(CCT - BAHIA BLANCA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - BAHIA BLANCA
Citación
Buitrago, Claudia Graciela; Arango, Nadia Soledad; Boland, Ricardo Leopoldo; 1α,25(OH)2D3-dependent modulation of Akt in proliferating and differentiating C2C12 skeletal muscle cells; Wiley-liss, Div John Wiley & Sons Inc; Journal of Cellular Biochemistry; 113; 8-2012; 1170-1181
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