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dc.contributor.author
Falcone Ferreyra, María Lorena
dc.contributor.author
Rodriguez, Eduardo Jose
dc.contributor.author
Casas, María Isabel
dc.contributor.author
Labadie, Guillermo Roberto
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Grotewold, Erich
dc.contributor.author
Casati, Paula
dc.date.available
2016-10-27T21:11:30Z
dc.date.issued
2013-10
dc.identifier.citation
Falcone Ferreyra, María Lorena; Rodriguez, Eduardo Jose; Casas, María Isabel; Labadie, Guillermo Roberto; Grotewold, Erich; et al.; Identification of a Bifunctional Maize C- and O-Glucosyltransferase; American Society For Biochemistry And Molecular Biology; Journal Of Biological Chemistry; 288; 44; 10-2013; 31678-31688
dc.identifier.issn
0021-9258
dc.identifier.uri
http://hdl.handle.net/11336/7832
dc.description.abstract
Flavonoids accumulate in plant vacuoles usually as O-glycosylated derivatives, but several species can also synthesize flavonoid C-glycosides. Recently, we demonstrated that a flavanone 2-hydroxylase (ZmF2H1, CYP93G5) converts flavanones to the corresponding 2-hydroxy derivatives, which are expected to serve as substrates for C-glycosylation. Here, we isolated a cDNA encoding a UDP-dependent glycosyltransferase (UGT708A6), and its activity was characterized by in vitro and in vivo bioconversion assays. In vitro assays using 2-hydroxyflavanones as substrates and in vivo activity assays in yeast co-expressing ZmF2H1 and UGT708A6 show the formation of the flavones C-glycosides. UGT708A6 can also O-glycosylate flavanones in bioconversion assays in Escherichia coli as well as by in vitro assays with the purified recombinant protein. Thus, UGT708A6 is a bifunctional glycosyltransferase that can produce both C- and O-glycosidated flavonoids, a property not previously described for any other glycosyltransferase.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Society For Biochemistry And Molecular Biology
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Flavonoids
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Glycosyltransferase
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Maize
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Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Identification of a Bifunctional Maize C- and O-Glucosyltransferase
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-14T12:49:12Z
dc.journal.volume
288
dc.journal.number
44
dc.journal.pagination
31678-31688
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Bethesda
dc.description.fil
Fil: Falcone Ferreyra, María Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
dc.description.fil
Fil: Rodriguez, Eduardo Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
dc.description.fil
Fil: Casas, María Isabel. The Ohio State University. Plant Biotechnology Center. Department Plant Cell Molecular Biology; Estados Unidos
dc.description.fil
Fil: Labadie, Guillermo Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Química Rosario; Argentina
dc.description.fil
Fil: Grotewold, Erich. The Ohio State University. Plant Biotechnology Center; Estados Unidos
dc.description.fil
Fil: Casati, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
dc.journal.title
Journal Of Biological Chemistry
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3814763/
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://www.jbc.org/content/288/44/31678.abstract
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1074/jbc.M113.510040
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