Artículo

A simply method for the addition of rotenone in Arabidopsis thaliana leaves

Maliandi, María VictoriaIcon ; Rius, Sebastian PabloIcon ; Busi, María VictoriaIcon ; Gomez Casati, Diego FabianIcon
Fecha de publicación: 07/2015
Editorial: Taylor & Francis
Revista: Plant Signaling and Behavior
ISSN: 1559-2316
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:

Resumen

A simple and reproducible method for the treatment of Arabidopsis thalianaleaves with rotenone is presented. Rosette leaves were incubated with rotenone andTriton X-100 for at least 15 h. Treated leaves showed increased expression of COX19 and BCS1a, two genes known to be induced in Arabidopsis cell cultures afterrotenone treatment. Moreover, rotenone/Triton X-100 incubated leaves presented aninhibition of oxygen uptake. The simplicity of the procedure shows this methodology useful for studying the effect of the addition of rotenone to a photosynthetic tissue in situ.
Palabras clave: Rotenone , Arabidopsis Thaliana , Mitochondria , Mitochondrial Respiration , Complex I
 
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/7826
URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4883935/
DOI: http://www.tandfonline.com/doi/full/10.1080/15592324.2015.1073871
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Articulos de CENTRO DE EST.FOTOSINTETICOS Y BIOQUIMICOS (I)
Citación
Maliandi, María Victoria; Rius, Sebastian Pablo; Busi, María Victoria; Gomez Casati, Diego Fabian; A simply method for the addition of rotenone in Arabidopsis thaliana leaves; Taylor & Francis; Plant Signaling and Behavior; 10; 10; 7-2015; 1-3
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