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dc.contributor.author
Lamas, Daniela Lorena
dc.contributor.author
Yeannes, Maria Isabel
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Massa, Agueda Elena
dc.date.available
2019-06-12T20:55:24Z
dc.date.issued
2017-07
dc.identifier.citation
Lamas, Daniela Lorena; Yeannes, Maria Isabel; Massa, Agueda Elena; Alkaline trypsin from the viscera and heads of Engraulis anchoita: Partial purification and characterization; Institute of Bioorganic Chemistry; Biotechnologia; 98; 2; 7-2017; 103-112
dc.identifier.issn
0860-7796
dc.identifier.uri
http://hdl.handle.net/11336/78152
dc.description.abstract
Marine by-products contain valuable protein fractions. To ensure a profitable utilization of wastes from the fish industry, the knowledge about their quality and composition is necessary. Fish digestive proteolytic enzymes from cold-adapted ectothermic organisms have found applications in several industries because their temperature requirements and other characteristics differ from those of homologous proteases from warm-blooded animals. Herein, we describe detection, partial purification, and characterization of proteolytic enzymes from the viscera and heads of Engraulis anchoita. Enzymatic activities of the by-products were assayed using azocaseín as a substrate. To characterize the alkali protein fraction, the activity against inhibitors and their molecular weights were studied. The crude protein extract exhibited maximal activity at pH 8.0 and 60EC. Results relative to the substrate-specific Nabenzoyl-DL-arginine-p-nitroanilide and the 25 kDa molecular weight indicated that the recovered protease was trypsin. The activity showed an increment in presence of SDS and a slight decrease when it was incubated with EDTA. Increasing the concentration of NaCl up to 5% did not significantly decrease the protein’s activity. The results obtained suggest that by-products of anchovy industry could be used in the detergents industry.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Institute of Bioorganic Chemistry
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Alkaline Proteases
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Engraulis Anchoita
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Heads
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Trypsin
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Viscera
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Agricultura
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Agricultura, Silvicultura y Pesca
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CIENCIAS AGRÍCOLAS
dc.title
Alkaline trypsin from the viscera and heads of Engraulis anchoita: Partial purification and characterization
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-05-15T18:12:29Z
dc.journal.volume
98
dc.journal.number
2
dc.journal.pagination
103-112
dc.journal.pais
Polonia
dc.description.fil
Fil: Lamas, Daniela Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Subsede Instituto Nacional de Investigación y Desarrollo Pesquero; Argentina
dc.description.fil
Fil: Yeannes, Maria Isabel. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
dc.description.fil
Fil: Massa, Agueda Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Subsede Instituto Nacional de Investigación y Desarrollo Pesquero; Argentina
dc.journal.title
Biotechnologia
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.5114/bta.2017.68309
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.biotechnologia-journal.org/Alkaline-trypsin-from-the-viscera-and-heads-of-Engraulis-anchoita-partial-purification-r-nand-characterization,85,30107,0,1.html