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dc.contributor.author
Soares, Raquel
dc.contributor.author
Meireles, Manuela
dc.contributor.author
Rocha, Ana
dc.contributor.author
Pirraco, Ana
dc.contributor.author
Obiol, Diego Javier
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dc.contributor.author
Alonso, Eliana Noelia
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dc.contributor.author
Joos, Gisela
dc.contributor.author
Balogh, Gabriela Andrea
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dc.date.available
2019-06-11T17:37:25Z
dc.date.issued
2011-06
dc.identifier.citation
Soares, Raquel; Meireles, Manuela; Rocha, Ana; Pirraco, Ana; Obiol, Diego Javier; et al.; Maitake (D Fraction) Mushroom Extract Induces Apoptosis in Breast Cancer Cells by BAK-1 Gene Activation; Mary Ann Liebert; Journal Of Medicinal Food; 14; 6; 6-2011; 563-572
dc.identifier.issn
1096-620X
dc.identifier.uri
http://hdl.handle.net/11336/77960
dc.description.abstract
For many years mushrooms have been used empirically in traditional medicine to treat several diseases. Study of the maitake mushroom, with its immunomodulatory and antitumoral properties, has led to the isolation of several bioactive compounds. One of these, D fraction, is known to reduce tumor cell viability. This study examined the effect of isolated D fraction on viability and apoptosis of human breast cancer cells (MCF7). These cells were treated with maitake (D fraction) extract at 18μg/mL, 36μg/mL, 91μg/mL, 183μg/mL, or 367μg/mL or were left untreated (control) for 24 hours. MCF7 incubation with the maitake extract resulted in decreased cell viability [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl) -2H-tetrazolium assay] in a dose-dependent manner. Apoptosis was statistically significantly increased in a dose-dependent manner at every concentration tested (terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling assay). Upon incubation with D fraction, a microarray assay revealed upregulation of BAK-1 and cytochrome c transcripts, 2 proteins directly involved in the apoptotic pathway. Reverse transcriptase polymerase chain reaction studies confirmed these findings; BAK-1 was one of most overexpressed gene, as observed by microarray assay. These findings confirm the apoptotic effect of maitake D fraction in breast cancer cells and further highlight the involvement of cytochrome c release to the cytoplasm. Cytoplasmic release of cytochrome c, another player in the apoptotic pathway, was also increased after incubation with D fraction in a dose-dependent manner. This finding indicates that the effect of this compound involves mitochondrial dysfunction. The identification of the molecular mechanisms by which D fraction exerts its effects is crucial for the development of preventive and therapeutic strategies for cancer.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Mary Ann Liebert
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Apoptosis
dc.subject
Bak-1
dc.subject
Breast Cancer
dc.subject
Cytochrome C
dc.subject
Maitake
dc.subject
Mushroom
dc.subject.classification
Bioquímica y Biología Molecular
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dc.subject.classification
Medicina Básica
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dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
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dc.title
Maitake (D Fraction) Mushroom Extract Induces Apoptosis in Breast Cancer Cells by BAK-1 Gene Activation
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-05-14T14:06:22Z
dc.identifier.eissn
1557-7600
dc.journal.volume
14
dc.journal.number
6
dc.journal.pagination
563-572
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Nueva York
dc.description.fil
Fil: Soares, Raquel. Universidad de Porto; Portugal
dc.description.fil
Fil: Meireles, Manuela. Universidad de Porto; Portugal
dc.description.fil
Fil: Rocha, Ana. Universidad de Porto; Portugal
dc.description.fil
Fil: Pirraco, Ana. Universidad de Porto; Portugal
dc.description.fil
Fil: Obiol, Diego Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
dc.description.fil
Fil: Alonso, Eliana Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
dc.description.fil
Fil: Joos, Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
dc.description.fil
Fil: Balogh, Gabriela Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
dc.journal.title
Journal Of Medicinal Food
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.liebertpub.com/doi/10.1089/jmf.2010.0095
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1089/jmf.2010.0095
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