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dc.contributor.author
Buzaleh, Ana Maria
dc.contributor.author
Moreno Carraledo, Maria
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Mendez, Manuel
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Batlle, Alcira Maria del C.
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Enriquez de Salamanca, Rafael
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Moran Jimenez, Maria Jose
dc.date.available
2016-10-24T20:39:09Z
dc.date.issued
2013-12
dc.identifier.citation
Buzaleh, Ana Maria; Moreno Carraledo, Maria; Mendez, Manuel; Batlle, Alcira Maria del C.; Enriquez de Salamanca, Rafael; et al.; Heme metabolism, oxidative and nitrosative markers in a mouse model of Hemochromatosis: Effect of Isoflurane, ethanol and 5-aminolevulinic acid; Bioinfo Publications; Journal of Pathology Research; 2; 1; 12-2013; 15-20
dc.identifier.issn
0976-8068
dc.identifier.uri
http://hdl.handle.net/11336/7792
dc.description.abstract
Hereditary hemochromatosis (HH) is characterized by iron homeostasis alterations. Association between HH and Porphyria Cutanea Tarda has been reported. The aim was to characterize oxidative and nitrosative stress status and its relationship with heme metabolism in a hemochromatosis mouse model (Hfe-/-), and to evaluate the effects of Isoflurane, ethanol and 5-aminolevulinic acid (ALA). Male and female Hfe−/− and wild-type C57BL/6J mice received Isoflurane (2 ml/kg); ethanol (30%) or ALA (40 mg/kg). In male Hfe-/-, reduced glutathione (GSH) was diminished respect to C57BL/6J mice. Female Hfe-/- showed higher levels of GSH and total antioxidant capacity than male Hfe-/-. Catalase activity was lower in male and female Hfe-/- than in controls. 5-Aminolevulinic acid synthetase activity was higher in male and female Hfe-/- than in controls. In male Hfe-/-, Porphobilinogen deaminase (PBG-D) activity was augmented and Heme oxygenase (HO) activity was diminished probably to avoid iron increase. Isoflurane and ethanol reduced PBG-D and increased HO activities. HO induction would indicate oxidative stress instauration being more striking due to ethanol that also induced Superoxide dismutase activity. Isoflurane reduced Nitric Oxide Synthase expression. ALA altered antioxidant system. In Hfe-/- mice different metabolisms were altered being more affected by the drugs studied. Findings here described would contribute to increase the knowledge about the association between HH and the Porphyrias and about the effects of volatile anaesthetics on different metabolisms in genetic models of Porphyrias and associated diseases.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Bioinfo Publications
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
Heme Biosynthesis
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Heme Oxygenase
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Oxidative Stress
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Volatile Anaesthetics
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Porphyrinogenic Drugs
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Otras Ciencias de la Salud
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Ciencias de la Salud
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Heme metabolism, oxidative and nitrosative markers in a mouse model of Hemochromatosis: Effect of Isoflurane, ethanol and 5-aminolevulinic acid
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-08-30T17:09:28Z
dc.identifier.eissn
0976-8076
dc.journal.volume
2
dc.journal.number
1
dc.journal.pagination
15-20
dc.journal.pais
India
dc.description.fil
Fil: Buzaleh, Ana Maria. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Centro de Invest. Sobre Porfirinas y Porfirias; Argentina. Hospital 12 de Octubre; España. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
dc.description.fil
Fil: Moreno Carraledo, Maria. Hospital 12 de Octubre; España
dc.description.fil
Fil: Mendez, Manuel. Hospital 12 de Octubre; España
dc.description.fil
Fil: Batlle, Alcira Maria del C.. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Centro de Invest. Sobre Porfirinas y Porfirias; Argentina
dc.description.fil
Fil: Enriquez de Salamanca, Rafael. Hospital 12 de Octubre; España
dc.description.fil
Fil: Moran Jimenez, Maria Jose. Hospital 12 de Octubre; España
dc.journal.title
Journal of Pathology Research
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.bioinfopublication.org/viewhtml.php?artid=BIA0002063
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.9735/0976-8068.2.1.15-20
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