Mostrar el registro sencillo del ítem
dc.contributor.author
Lavandera, Jimena Veronica
dc.contributor.author
Ruspini, Silvina Fernanda
dc.contributor.author
Batlle, Alcira Maria del C.
dc.contributor.author
Buzaleh, Ana Maria
dc.date.available
2016-10-24T20:00:32Z
dc.date.issued
2015-02
dc.identifier.citation
Lavandera, Jimena Veronica; Ruspini, Silvina Fernanda; Batlle, Alcira Maria del C.; Buzaleh, Ana Maria; Cytochrome P450 expression in mouse brain: specific isoenzymes involved in Phase I metabolizing system of porphyrinogenic agents in both microsomes and mitochondria; Natl Research Council Canada-n R C Research Press; Biochemistry And Cell Biology; 93; 1; 2-2015; 102-107
dc.identifier.issn
0829-8211
dc.identifier.uri
http://hdl.handle.net/11336/7783
dc.description.abstract
Brain cytochrome P450 (CYP) metabolizes a variety of drugs to produce their pharmacological effects within the brain. We have previously observed that porphyrinogenic agents altered CYP levels in brain. The aim of this work was to further study the involvement of mice brain mitochondrial and microsomal Phase I drug metabolizing system when porphyrinogenic agents, such as Enflurane, isoflurane, allylisopropylacetamide, veronal, ethanol, and Griseofulvin were administered. To this end, CYP2E1, CYP2B1, and CYP3A4 expression were measured. NADPH cytochrome P450 reductase (CPR) expression was also determined. Western Blots were performed in microsomes and mitochondria of whole brain. Some of the drugs studied altered expression mainly in microsomes. Chronic Isoflurane augmented mitochondrial isoform, although this anaesthetic diminished microsomal expression. Ethanol and topical Griseofulvin affected expression in microsomes but not in mitochondria. CYP2E1 mitochondrial activity was induced by acute Enflurane; while the activity of the microsomal protein was enhanced in alcoholised animals. Ethanol also induced CYP2E1 expression in microsomes, although Isoflurane provoked opposite effects in mitochondria and microsomes. Expression of CPR was also induced. Several reports support an emergent role of CYP enzymes in the pathogenesis of neurological disorders, so CYP response in brain could be one of the multiples factors influencing porphyria acute attacks.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Natl Research Council Canada-n R C Research Press
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Cytochrome P450
dc.subject
Brain
dc.subject
Porphyrinogenic Agents
dc.subject
Volatile Anaesthetics
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Cytochrome P450 expression in mouse brain: specific isoenzymes involved in Phase I metabolizing system of porphyrinogenic agents in both microsomes and mitochondria
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-08-30T17:12:20Z
dc.journal.volume
93
dc.journal.number
1
dc.journal.pagination
102-107
dc.journal.pais
Canadá
dc.journal.ciudad
Otawa
dc.description.fil
Fil: Lavandera, Jimena Veronica. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
dc.description.fil
Fil: Ruspini, Silvina Fernanda. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Centro de Invest. Sobre Porfirinas y Porfirias; Argentina
dc.description.fil
Fil: Batlle, Alcira Maria del C.. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Centro de Invest. Sobre Porfirinas y Porfirias; Argentina
dc.description.fil
Fil: Buzaleh, Ana Maria. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Quimica Biologica. Laboratorio de Metabolismo D/hemo. Porfirinas y Enf.relac.; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Centro de Invest. Sobre Porfirinas y Porfirias; Argentina
dc.journal.title
Biochemistry And Cell Biology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.nrcresearchpress.com/doi/abs/10.1139/bcb-2014-0088?url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org&rfr_dat=cr_pub%3Dpubmed&#.WA5IZfnhCJA
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1139/bcb-2014-0088
Archivos asociados