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dc.contributor.author
Pizzurro, Gabriela Andrea
dc.contributor.author
Madorsky Rowdo, Florencia Paula
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Pujol Lereis, Luciana Mercedes
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Quesada Allue, Luis Alberto
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Copati, Andrea M.
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Roberti, Maria Paula
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Teillaud, Jean Luc
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Levy, Estrella Mariel
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Barrio, Maria Marcela
dc.contributor.author
Mordoh, Jose
dc.date.available
2016-10-24T17:03:03Z
dc.date.issued
2013-01
dc.identifier.citation
Pizzurro, Gabriela Andrea; Madorsky Rowdo, Florencia Paula; Pujol Lereis, Luciana Mercedes; Quesada Allue, Luis Alberto; Copati, Andrea M.; et al.; High lipid content of irradiated human melanoma cells does not affect cytokine-matured dendritic cell function; Springer; Cancer Immunology Immunotherapy; 62; 1; 1-2013; 3-15
dc.identifier.issn
0340-7004
dc.identifier.uri
http://hdl.handle.net/11336/7776
dc.description.abstract
Gamma irradiation is one of the methods used to sterilize melanoma cells prior to coculturing them with monocyte-derived immature dendritic cells in order to develop antitumor vaccines. However, the changes taking place in tumor cells after irradiation and their interaction with dendritic cells have been scarcely analyzed. We demonstrate here for the first time that after irradiation a fraction of tumor cells present large lipid bodies, which mainly contain triglycerides that are several-fold increased as compared to viable cells as determined by staining with Oil Red O and BODIPY 493/503 and by biochemical analysis. Phosphatidyl-choline, phosphatidyl-ethanolamine and sphingomyelin are also increased in the lipid bodies of irradiated cells. Lipid bodies do not contain the melanoma-associated antigen MART-1. After coculturing immature dendritic cells with irradiated melanoma cells, tumor cells tend to form clumps to which dendritic cells adhere. Under such conditions, dendritic cells are unable to act as stimulating cells in a mixed leukocyte reaction. However, when a maturation cocktail composed of TNF-alpha, IL-6, IL-1beta and prostaglandin E2 is added to the coculture, the tumor cells clumps disaggregate, dendritic cells remain free in suspension and their ability to efficiently stimulate allogeneic lymphocytes is restored. These results help to understand the events following melanoma cell irradiation, shed light about interactions between irradiated cells and dendritic cells, and may help to develop optimized dendritic cell vaccines for cancer therapy.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.title
High lipid content of irradiated human melanoma cells does not affect cytokine-matured dendritic cell function
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-09-05T13:18:32Z
dc.journal.volume
62
dc.journal.number
1
dc.journal.pagination
3-15
dc.journal.pais
Alemania
dc.journal.ciudad
Berlin
dc.description.fil
Fil: Pizzurro, Gabriela Andrea. Fundacion Cancer. Centro de Investigaciones Oncologicas; Argentina
dc.description.fil
Fil: Madorsky Rowdo, Florencia Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
dc.description.fil
Fil: Pujol Lereis, Luciana Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
dc.description.fil
Fil: Quesada Allue, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
dc.description.fil
Fil: Copati, Andrea M.. Fundacion Cancer. Centro de Investigaciones Oncologicas; Argentina
dc.description.fil
Fil: Roberti, Maria Paula. Fundacion Cancer. Centro de Investigaciones Oncologicas; Argentina
dc.description.fil
Fil: Teillaud, Jean Luc. Universite Pierre et Marie Curie; Francia
dc.description.fil
Fil: Levy, Estrella Mariel. Fundacion Cancer. Centro de Investigaciones Oncologicas; Argentina
dc.description.fil
Fil: Barrio, Maria Marcela. Fundacion Cancer. Centro de Investigaciones Oncologicas; Argentina
dc.description.fil
Fil: Mordoh, Jose. Fundacion Cancer. Centro de Investigaciones Oncologicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
dc.journal.title
Cancer Immunology Immunotherapy
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007/s00262-012-1295-4
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s00262-012-1295-4
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