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dc.contributor.author
Frungieri, Monica Beatriz
dc.contributor.author
Albrecht, Martín
dc.contributor.author
Raemsch, Romi
dc.contributor.author
Mayerhofer, Artur
dc.date.available
2019-05-31T17:59:04Z
dc.date.issued
2005-04
dc.identifier.citation
Frungieri, Monica Beatriz; Albrecht, Martín; Raemsch, Romi; Mayerhofer, Artur; The action of the mast cell product tryptase on cyclooxygenase-2 (COX2) and subsequent fibroblast proliferation involves activation of the extracellular signal-regulated kinase isoforms 1 and 2 (erk1/2); Elsevier Science Inc; Cellular Signalling; 17; 4; 4-2005; 525-533
dc.identifier.issn
0898-6568
dc.identifier.uri
http://hdl.handle.net/11336/77465
dc.description.abstract
The mast cell product tryptase, via protease-activated receptor 2 (PAR2), induces cyclooxygenase-2 (COX2) and 15-deoxy-prostaglandin J2 (15d-PGJ2) synthesis. 15d-PGJ2, through the nuclear peroxisome proliferator activated receptor gamma (PPARγ), subsequently causes fibroblast proliferation. In this study we attempted to determine initial events of the tryptase/PAR2 signaling pathway leading to COX2 induction and fibroblast proliferation. In human fibroblasts (HFFF2), cDNA array, RT-PCR and Western blotting studies demonstrated that tryptase, but not 15d-PGJ2, up-regulates c-jun, c-fos and COX2 expression, and phosphorylates the extracellular signal-regulated kinase isoforms 1 and 2 (erk1/2). Furthermore, tryptase effects on erk1/2, c-jun, c-fos, COX2 and cell proliferation were prevented by PD98059, an inhibitor of the mitogen-activated protein kinase kinase (MEK). Other kinases [P38, stress-activated protein kinase/c-jun N-terminal kinase (SAPK/JUNK), erk5], intracellular Ca 2+ or cAMP were not affected by tryptase/PAR2. Our study identifies crucial intracellular events leading to induction of COX2 and fibroblast proliferation, i.e. a cornerstone of fibrosis. © 2004 Elsevier Inc. All rights reserved.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science Inc
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Ca 2+
dc.subject
Camp
dc.subject
Cyclooxygenase-2
dc.subject
Map Kinases
dc.subject
Transcription Factors
dc.subject
Tryptase
dc.subject.classification
Inmunología
dc.subject.classification
Medicina Básica
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
The action of the mast cell product tryptase on cyclooxygenase-2 (COX2) and subsequent fibroblast proliferation involves activation of the extracellular signal-regulated kinase isoforms 1 and 2 (erk1/2)
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-05-14T15:51:06Z
dc.journal.volume
17
dc.journal.number
4
dc.journal.pagination
525-533
dc.journal.pais
Irlanda
dc.journal.ciudad
Shannon
dc.description.fil
Fil: Frungieri, Monica Beatriz. Ludwig Maximilians Universitat; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil
Fil: Albrecht, Martín. Ludwig Maximilians Universitat; Alemania
dc.description.fil
Fil: Raemsch, Romi. Ludwig Maximilians Universitat; Alemania
dc.description.fil
Fil: Mayerhofer, Artur. Ludwig Maximilians Universitat; Alemania
dc.journal.title
Cellular Signalling
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.cellsig.2004.09.017
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0898656804002074
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