Artículo
First identification of nosema ceranae (Microsporidia) infecting apis mellifera in Venezuela
Porrini, Leonardo Pablo
; Porrini, Martín Pablo
; Garrido, Paula Melisa
; Principal, Judith; Barrios Suarez, Carlos J.; Bianchi, Brigitte; Fernandez Iriarte, Pedro Jose
; Eguaras, Martin Javier
Fecha de publicación:
06/2017
Editorial:
Research Inst Pomology Floriculture
Revista:
Journal Of Apicultural Science
ISSN:
1643-4439
e-ISSN:
2299-4831
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Nosema ceranae is a pathogen of Apis mellifera, which seems to have jumped from its original host Asiatic honey bee Apis ceranae. Nosemosis which affects the honey bee Apis mellifera is caused by two parasitic fungi described as etiologic agents of the disease. Nosema apis was the only microsporidian infection identified in A. mellifera until N. ceranae in Taiwan and Europe. Nosema spp. positive samples of adult worker bees from the Venezuelean state of Lara were determined through light microscopy of spores. Samples were then tested to determine Nosema species (N.apis/N.ceranae) using previously reported PCR primers for the 16S rRNA gene. A multiplex PCR assay was used to differentiate both N. apis and N. ceranae species. Only N. ceranae was found in the analyzed samples and the percentage of infected foragers fluctuated between 18% and 60%.
Palabras clave:
Honeybee
,
Microsporidian
,
Multiplex Pcr
,
Nosema Apis
,
Nosema Ceranae
,
Venezuela
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Licencia
Identificadores
Colecciones
Articulos(CCT - MAR DEL PLATA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - MAR DEL PLATA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - MAR DEL PLATA
Citación
Porrini, Leonardo Pablo; Porrini, Martín Pablo; Garrido, Paula Melisa; Principal, Judith; Barrios Suarez, Carlos J.; et al.; First identification of nosema ceranae (Microsporidia) infecting apis mellifera in Venezuela; Research Inst Pomology Floriculture; Journal Of Apicultural Science; 61; 1; 6-2017; 149-152
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