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dc.contributor.author
Di Giorgio, Noelia Paula
dc.contributor.author
Catalano, Paolo Nicolás
dc.contributor.author
Lopez, Paula Veronica
dc.contributor.author
Gonzalez, Betina
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Semaan, Sheila J.
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Lopez, Gabriela Carina
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Kauffman, Alexander S.
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Rulli, Susana Beatriz
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Somoza, Gustavo Manuel
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Bettler, Bernhard
dc.contributor.author
Libertun, Carlos
dc.contributor.author
Lux, Victoria Adela R.
dc.date.available
2016-09-19T20:07:43Z
dc.date.issued
2013-12-31
dc.identifier.citation
Di Giorgio, Noelia Paula; Catalano, Paolo Nicolás; Lopez, Paula Veronica; Gonzalez, Betina; Semaan, Sheila J.; et al.; Lack of functional GABAB receptors alters Kiss1 , Gnrh1 and Gad1 mRNA expression in the medial basal hypothalamus at postnatal day 4; Karger; Neuroendocrinology; 98; 3; 31-12-2013; 212-223
dc.identifier.issn
0028-3835
dc.identifier.uri
http://hdl.handle.net/11336/7667
dc.description.abstract
Adult mice lacking functional GABA B receptors (GABA B1 KO) show altered Gnrh1 and Gad1 expressions in the preoptic area-anterior hypothalamus (POA-AH) and females display disruption of cyclicity and fertility. Here we addressed whether sexual differentiation of the brain and the proper wiring of the GnRH and kisspeptin systems were already disturbed in postnatal day 4 (PND4) GABA B1 KO mice. Methods: PND4 wild-type (WT) and GABA B1 KO mice of both sexes were sacrificed; tissues were collected to determine mRNA expression (qPCR), amino acids (HPLC), and hormones (RIA and/or IHC). Results: GnRH neuron number (IHC) did not differ among groups in olfactory bulbs or OVLT-POA. Gnrh1 mRNA (qPCR) in POA-AH was similar among groups. Gnrh1 mRNA in medial basal hypothalamus (MBH) was similar in WTs but was increased in GABA B1 KO females compared to GABA B1 KO males. Hypothalamic GnRH (RIA) was sexually different in WTs (males > females), but this sex difference was lost in GABA B1 KOs; the same pattern was observed when analyzing only the MBH, but not in the POA-AH. Arcuate nucleus Kiss1 mRNA (micropunch-qPCR) was higher in WT females than in WT males and GABA B1 KO females. Gad1 mRNA in MBH was increased in GABA B1 KO females compared to GABA B1 KO males. Serum LH and gonadal estradiol content were also increased in GABA B1 KOs. Conclusion: We demonstrate that GABA B Rs participate in the sexual differentiation of the ARC/MBH, because sex differences in several reproductive genes, such as Gad1 , Kiss1 and Gnrh1, are critically disturbed in GABA B1 KO mice at PND4, probably altering the organization and development of neural circuits governing the reproductive axis.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Karger
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Gaba B Receptors
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Gnrh
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Gaba
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Kiss 1 Protein
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Gonadotropins
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Sex Steroids
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Fisiología
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Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
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Neurociencias
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Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Lack of functional GABAB receptors alters Kiss1 , Gnrh1 and Gad1 mRNA expression in the medial basal hypothalamus at postnatal day 4
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-05-10T13:46:14Z
dc.identifier.eissn
1423-0194
dc.journal.volume
98
dc.journal.number
3
dc.journal.pagination
212-223
dc.journal.pais
Suiza
dc.journal.ciudad
Basel
dc.description.fil
Fil: Di Giorgio, Noelia Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.description.fil
Fil: Catalano, Paolo Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
dc.description.fil
Fil: Lopez, Paula Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
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Fil: Gonzalez, Betina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.description.fil
Fil: Semaan, Sheila J.. University Of California At Davis; Estados Unidos
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Fil: Lopez, Gabriela Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Investigaciones Biotecnológicas - Instituto Tecnológico Chascomús. Instituto de Investigaciones Biotecnológicas (sede Chascomús); Argentina
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Fil: Kauffman, Alexander S.. University Of California At San Diego; Estados Unidos
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Fil: Rulli, Susana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.description.fil
Fil: Somoza, Gustavo Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Investigaciones Biotecnológicas - Instituto Tecnológico Chascomús. Instituto de Investigaciones Biotecnológicas (sede Chascomús); Argentina
dc.description.fil
Fil: Bettler, Bernhard. University of Basel; Suiza
dc.description.fil
Fil: Libertun, Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
dc.description.fil
Fil: Lux, Victoria Adela R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.journal.title
Neuroendocrinology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1159/000355631
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.karger.com/Article/FullText/355631


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