Artículo
Acetaldehyde content and oxidative stress in the deleterious effects of alcohol drinking on rat uterine horn
Buthet, Lara Romina; Maciel, Maria Eugenia; Quintans, Leandro; Rodríguez De Castro, Carmen; Costantini, Martin Hernan; Fanelli, Silvia Laura
; Castro, Jose Alberto
; Castro, Gerardo Daniel
Fecha de publicación:
11/2013
Editorial:
Hindawi Publishing Corporation
Revista:
Journal of Toxicology
ISSN:
1687-8191
e-ISSN:
1687-8205
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
After alcohol exposure through a standard Lieber and De Carli diet for 28 days, a severe atrophy in the rat uteirne horn was observed, accompanied by significant alterations in its epithelial cells. Microsomal pathway of acetaldehyde production was slightly increased. Hydroxyl radicals were detected in the cytosolic fraction, and this was attributed to participation of xanthine oxidoreductase. They were also observed in the microsomal fraction in the presence of NADPH generating system. No generation of 1-hydroxyethyl was evidenced. The t-butylhydroperoxide-induced chemiluminescence analysis of uterine horn homogenates revealed a significant increase in the chemiluminiscence emission due to ethanol exposure. In the animals repeatedly exposed to alcohol, sulfhydryl content from uterine horn proteins was decreased, but no significant changes were observed in the protein carbonyl content from the same samples. Minor but significant decreasing changes were observed in the GSH content accompanied by a tendency to decrease in the GSH/GSSG ratio. A highly significant finding was the diminished activity content of glutathione peroxidase. Results suggest that acetaldehyde accumulation plus the oxidative stress may play an additional effect to the alcohol-promoted hormonal changes in the uterus reported by others after chronic exposure to alcohol. © 2013 Lara Romina Buthet et al.
Palabras clave:
Uterus
,
Ethanol
,
Acetaldehyde
,
Oxidative Stress
,
Reproductive Toxicity
,
Alcohol
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(ILPLA)
Articulos de INST.DE LIMNOLOGIA "DR. RAUL A. RINGUELET"
Articulos de INST.DE LIMNOLOGIA "DR. RAUL A. RINGUELET"
Articulos(UNIDEF)
Articulos de UNIDAD DE INVESTIGACION Y DESARROLLO ESTRATEGICOS PARA LA DEFENSA
Articulos de UNIDAD DE INVESTIGACION Y DESARROLLO ESTRATEGICOS PARA LA DEFENSA
Citación
Buthet, Lara Romina; Maciel, Maria Eugenia; Quintans, Leandro; Rodríguez De Castro, Carmen; Costantini, Martin Hernan; et al.; Acetaldehyde content and oxidative stress in the deleterious effects of alcohol drinking on rat uterine horn; Hindawi Publishing Corporation; Journal of Toxicology; 2013; 11-2013; 1-12
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