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Artículo

Global conformations of proteins as predicted from the modeling of their CZE mobility data

Deiber, Julio AlcidesIcon ; Piaggio, María Virginia; Peirotti, Marta BeatrizIcon
Fecha de publicación: 10/2011
Editorial: Wiley VCH Verlag
Revista: Electrophoresis
ISSN: 0173-0835
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
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Resumen

Estimations of protein global conformations in well-specified physicochemical microenvironments are obtained through global structural parameters defined from polypeptide-scale analyses. For this purpose protein electrophoretic mobility data must be interpreted through a physicochemical CZE model to obtain estimates of protein equivalent hydrodynamic radius, effective and total charge numbers, hydration, actual ionizing pK and pH-near molecule. The electrical permittivity of protein domain is also required. In this framework, the solvent drag on proteins is obtained via the characteristic friction power coefficient associated with the number of amino acid residues defining the global chain conformation in solution. Also, the packing dimension related to the spatial distribution of amino acid residues within the protein domain is evaluated and discussed. These scaling coefficients together with the effective and total charge number fractions of proteins provide relevant interpretations of protein global conformations mainly from collapsed globule to hybrid chain regimes. Also, protein transport properties may be estimated within this framework. In this regard, the central role played by the friction power coefficient in the evaluation of these properties is highlighted.
Palabras clave: Chain Packing Dimension , Effective Protein Charge Number Fraction , Electrophoretic Mobility Modeling , Friction Power Coefficient , Protein Global Conformations
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
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URI: http://hdl.handle.net/11336/76393
DOI: http://dx.doi.org/10.1002/elps.201100016
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Articulos(INTEC)
Articulos de INST.DE DES.TECNOL.PARA LA IND.QUIMICA (I)
Citación
Deiber, Julio Alcides; Piaggio, María Virginia; Peirotti, Marta Beatriz; Global conformations of proteins as predicted from the modeling of their CZE mobility data; Wiley VCH Verlag; Electrophoresis; 32; 20; 10-2011; 2779-2787
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