Show simple item record

dc.contributor.author Panero, Julieta
dc.contributor.author Stanganelli, Carmen
dc.contributor.author Arbelbide, Jorge
dc.contributor.author Fantl, Dorotea Beatriz
dc.contributor.author Kohan, Dana
dc.contributor.author Garcia Rivello, Hernan
dc.contributor.author Rabinovich, Gabriel Adrian
dc.contributor.author Slavutsky, Irma Rosa
dc.date.available 2016-09-14T19:30:46Z
dc.date.issued 2014-02
dc.identifier.citation Panero, Julieta; Stanganelli, Carmen; Arbelbide, Jorge; Fantl, Dorotea Beatriz; Kohan, Dana; et al.; Expression profile of shelterin components in plasma cell disorders. Clinical significance of POT1 overexpression; Elsevier; Blood Cells Molecules And Diseases; 52; 2-3; 2-2014; 134-139
dc.identifier.issn 1079-9796
dc.identifier.uri http://hdl.handle.net/11336/7635
dc.description.abstract In this study we analyzed the expression profile and the interactions of all shelterin components in patients with MM and MGUS, and correlated the results with hTERT expression, telomere length (TL) and clinical characteristics of the diseases. Bone marrow samples of 154 patients: 70 with MGUS and 84 with MM were studied. Gene expression was determined by real-time quantitative RT-PCR and TL was evaluated by terminal restriction fragments. A significant overexpression of TRF2, POT1 and hTERT in MM compared to MGUS was observed (p<0.03). Due to the great heterogeneity in hTERT levels, patients were divided into three groups of expression: GI (low), GII (intermediate) and GIII (high). This classification showed an upregulation of TRF2 and RAP1 in MGUS (GIII vs. GI: p<0.01), and an increased expression of TRF2 and TPP1 in MM (GIII vs. GI and GII: p<0.04). In addition, a significant over-expression of LGALS1 (encoding galectin-1, a multifunctional β-galactoside binding protein) was observed in GII/III respect to GI (p=0.013). TL was inversely correlated with telomerase expression (GII vs. GI: p=0.02). In MM, POT1 were positively correlated with β2microglobulin (β2M), advanced DS stages, high calcium levels and bone lesions (p=0.009), whereas TL was correlated positively with hemoglobin levels and negatively with β2M (p=0.02). Our findings provide the first evidence of a global modification in the expression of the six shelterin subunits, their interactions and the correlation between hTERT and LSGAL1 in plasma cell disorders. The strong association of POT1 with different parameters of adverse clinical outcome suggests its importance as a target for novel therapeutic strategies.
dc.format application/pdf
dc.language.iso eng
dc.publisher Elsevier
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject MULTIPLE MYELOMA
dc.subject MGUS
dc.subject TELOMERE-ASSOCIATED GENES
dc.subject POT1 EXPRESSION
dc.subject.classification Genética Humana
dc.subject.classification Medicina Básica
dc.subject.classification CIENCIAS MÉDICAS Y DE LA SALUD
dc.title Expression profile of shelterin components in plasma cell disorders. Clinical significance of POT1 overexpression
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2016-06-15T19:12:50Z
dc.journal.volume 52
dc.journal.number 2-3
dc.journal.pagination 134-139
dc.journal.pais Estados Unidos
dc.journal.ciudad Nueva York
dc.description.fil Fil: Panero, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental; Argentina
dc.description.fil Fil: Stanganelli, Carmen. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "mariano R. Castex"; Argentina
dc.description.fil Fil: Arbelbide, Jorge. Hospital Italiano de Buenos Aires; Argentina
dc.description.fil Fil: Fantl, Dorotea Beatriz. Hospital Italiano de Buenos Aires; Argentina
dc.description.fil Fil: Kohan, Dana. Hospital Italiano de Buenos Aires; Argentina
dc.description.fil Fil: Garcia Rivello, Hernan. Hospital Italiano de Buenos Aires; Argentina
dc.description.fil Fil: Rabinovich, Gabriel Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
dc.description.fil Fil: Slavutsky, Irma Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental; Argentina
dc.journal.title Blood Cells Molecules And Diseases
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1079979613002313
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.bcmd.2013.10.002


Archivos asociados

Icon
Blocked Acceso no disponible

This item appears in the following Collection(s)

Show simple item record

info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Atribución-NoComercial-SinDerivadas 2.5 Argentina (CC BY-NC-ND 2.5 AR)