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dc.contributor.author
Panero, Julieta
dc.contributor.author
Stanganelli, Carmen
dc.contributor.author
Arbelbide, Jorge
dc.contributor.author
Fantl, Dorotea Beatriz
dc.contributor.author
Kohan, Dana
dc.contributor.author
Garcia Rivello, Hernan
dc.contributor.author
Rabinovich, Gabriel Adrian
dc.contributor.author
Slavutsky, Irma Rosa
dc.date.available
2016-09-14T19:30:46Z
dc.date.issued
2014-02
dc.identifier.citation
Panero, Julieta; Stanganelli, Carmen; Arbelbide, Jorge; Fantl, Dorotea Beatriz; Kohan, Dana; et al.; Expression profile of shelterin components in plasma cell disorders. Clinical significance of POT1 overexpression; Elsevier; Blood Cells Molecules And Diseases; 52; 2-3; 2-2014; 134-139
dc.identifier.issn
1079-9796
dc.identifier.uri
http://hdl.handle.net/11336/7635
dc.description.abstract
In this study we analyzed the expression profile and the interactions of all shelterin components in patients with MM and MGUS, and correlated the results with hTERT expression, telomere length (TL) and clinical characteristics of the diseases. Bone marrow samples of 154 patients: 70 with MGUS and 84 with MM were studied. Gene expression was determined by real-time quantitative RT-PCR and TL was evaluated by terminal restriction fragments. A significant overexpression of TRF2, POT1 and hTERT in MM compared to MGUS was observed (p<0.03). Due to the great heterogeneity in hTERT levels, patients were divided into three groups of expression: GI (low), GII (intermediate) and GIII (high). This classification showed an upregulation of TRF2 and RAP1 in MGUS (GIII vs. GI: p<0.01), and an increased expression of TRF2 and TPP1 in MM (GIII vs. GI and GII: p<0.04). In addition, a significant over-expression of LGALS1 (encoding galectin-1, a multifunctional β-galactoside binding protein) was observed in GII/III respect to GI (p=0.013). TL was inversely correlated with telomerase expression (GII vs. GI: p=0.02). In MM, POT1 were positively correlated with β2microglobulin (β2M), advanced DS stages, high calcium levels and bone lesions (p=0.009), whereas TL was correlated positively with hemoglobin levels and negatively with β2M (p=0.02). Our findings provide the first evidence of a global modification in the expression of the six shelterin subunits, their interactions and the correlation between hTERT and LSGAL1 in plasma cell disorders. The strong association of POT1 with different parameters of adverse clinical outcome suggests its importance as a target for novel therapeutic strategies.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Multiple Myeloma
dc.subject
Mgus
dc.subject
Telomere-Associated Genes
dc.subject
Pot1 Expression
dc.subject.classification
Genética Humana
dc.subject.classification
Medicina Básica
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Expression profile of shelterin components in plasma cell disorders. Clinical significance of POT1 overexpression
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-06-15T19:12:50Z
dc.journal.volume
52
dc.journal.number
2-3
dc.journal.pagination
134-139
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Nueva York
dc.description.fil
Fil: Panero, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental; Argentina
dc.description.fil
Fil: Stanganelli, Carmen. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "mariano R. Castex"; Argentina
dc.description.fil
Fil: Arbelbide, Jorge. Hospital Italiano de Buenos Aires; Argentina
dc.description.fil
Fil: Fantl, Dorotea Beatriz. Hospital Italiano de Buenos Aires; Argentina
dc.description.fil
Fil: Kohan, Dana. Hospital Italiano de Buenos Aires; Argentina
dc.description.fil
Fil: Garcia Rivello, Hernan. Hospital Italiano de Buenos Aires; Argentina
dc.description.fil
Fil: Rabinovich, Gabriel Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
dc.description.fil
Fil: Slavutsky, Irma Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental; Argentina
dc.journal.title
Blood Cells Molecules And Diseases
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1079979613002313
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.bcmd.2013.10.002


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