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dc.contributor.author
Deiber, Julio Alcides
dc.contributor.author
Piaggio, María Virginia
dc.contributor.author
Peirotti, Marta Beatriz
dc.date.available
2019-05-15T15:28:20Z
dc.date.issued
2013-03
dc.identifier.citation
Deiber, Julio Alcides; Piaggio, María Virginia; Peirotti, Marta Beatriz; Determination of electrokinetic and hydrodynamic parameters of proteins by modeling their electrophoretic mobilities through the electrically charged spherical porous particle; Wiley VCH Verlag; Electrophoresis; 34; 5; 3-2013; 700-707
dc.identifier.issn
0173-0835
dc.identifier.uri
http://hdl.handle.net/11336/76349
dc.description.abstract
This work explores the possibility of using the electrically charged "spherical porous particle" (SPP) to model the electrophoretic mobility of proteins in the low charge regime. In this regard, the electrophoretic mobility expression of the charged SPP (Hermans-Fujita model) is used and applied here to BSA and staphylococcal nuclease for different protocol pH values. The SPP is presented within the general framework of the "spherical soft particle" as described in the literature. The physicochemical conditions required to model proteins as SPP from their experimentally determined electrophoretic mobilities are established. It is shown that particle permeability and porosity and chain packing and friction fractal dimensions are relevant structural properties of proteins when hydrodynamic interaction between amino acid residues is present. The charge regulation phenomenon of BSA and staphylococcal nuclease with pIs ≈ 5.71 and 9.63, respectively, is described through the SPP within a wide range of bulk pH values. These case studies illustrate when the average regulating (pH) of the protein domain is lower and higher than the protocol pH. Further research for using the general spherical soft particle is also proposed on the basis of results and main conclusions.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley VCH Verlag
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Polyampholyte Spherical Porous Particle
dc.subject
Polyampholyte Spherical Soft Particle
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Polypeptide Permeability
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Protein Electrical Charge Regulation
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Protein Electrophoretic Mobility
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Otras Ingeniería Química
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Ingeniería Química
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INGENIERÍAS Y TECNOLOGÍAS
dc.title
Determination of electrokinetic and hydrodynamic parameters of proteins by modeling their electrophoretic mobilities through the electrically charged spherical porous particle
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-05-14T17:51:07Z
dc.journal.volume
34
dc.journal.number
5
dc.journal.pagination
700-707
dc.journal.pais
Alemania
dc.journal.ciudad
Weinheim
dc.description.fil
Fil: Deiber, Julio Alcides. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
dc.description.fil
Fil: Piaggio, María Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
dc.description.fil
Fil: Peirotti, Marta Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
dc.journal.title
Electrophoresis
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/elps.201200405
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