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Artículo

Prenatal restraint stress decreases the expression of Alpha-7 Nicotinic receptor in the brain of adult rat offspring

Baier, Carlos JavierIcon ; Pallares, Maria EugeniaIcon ; Adrover, EzequielaIcon ; Monteleone, Melisa CarolinaIcon ; Brocco, Marcela AdrianaIcon ; Barrantes, Francisco JoseIcon ; Antonelli, Marta CristinaIcon
Fecha de publicación: 02/2015
Editorial: Taylor & Francis Ltd
Revista: Stress
ISSN: 1025-3890
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Drogadicción

Resumen

Prenatal stress (PS) strongly impacts fetal brain development and function in adulthood. In normal aging and Alzheimer's disease, there is hypothalamic–pituitary–adrenal axis dysfunction and loss of cholinergic neurons and neuronal nicotinic acetylcholine receptors (nAChRs). This study investigated whether prenatal restraint stress affects nAChR expression in the brain of adult offspring. For PS, pregnant dams were placed in a plastic restrainer for 45 min, three times daily during the last week of pregnancy; controls were undisturbed. Male offspring were analyzed at postnatal day (PND) 60 (n = 4 rats per group). Western blot (WB) and fluorescence microscopy showed that PS decreased α7-AChR subunit expression (∼50%) in the frontal cortex in the adult offspring. PS decreased significantly the number of α7-AChR-expressing cells in the medial prefrontal cortex (by ∼25%) and in the sensory-motor cortex (by ∼20%) without affecting the total cell number in those areas. No alterations were found in the hippocampus by quantitative polymerase chain reaction (qPCR), or WB analysis, but a detailed fluorescence microscopy analysis showed that PS affected α7-AChR mainly in the CA3 and dentate gyrus subfields: PS decreased α7-AChR subunit expression by ∼25 and ∼30%, respectively. Importantly, PS decreased the number of α7-AChR-expressing cells and the total cell number (by ∼15 and 20%, respectively) in the dentate gyrus. PS differently affected α4-AChR: PS impaired its mRNA expression in the frontal cortex (by ∼50%), without affecting protein levels. These results demonstrate that disturbances during gestation produce long-term alterations in the expression pattern of α7-AChR in rat brain.
Palabras clave: Aging , Cholinergic , Cortex , Gestational Stress , Hippocampus , Neurodegenerattion
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/7630
URL: http://www.tandfonline.com/doi/full/10.3109/10253890.2015.1022148
DOI: http://dx.doi.org/10.3109/10253890.2015.1022148
Colecciones
Articulos(IIB-INTECH)
Articulos de INST.DE INVEST.BIOTECNOLOGICAS - INSTITUTO TECNOLOGICO CHASCOMUS
Articulos(INIBIBB)
Articulos de INST.DE INVEST.BIOQUIMICAS BAHIA BLANCA (I)
Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Citación
Baier, Carlos Javier; Pallares, Maria Eugenia; Adrover, Ezequiela; Monteleone, Melisa Carolina; Brocco, Marcela Adriana; et al.; Prenatal restraint stress decreases the expression of Alpha-7 Nicotinic receptor in the brain of adult rat offspring; Taylor & Francis Ltd; Stress; 18; 4; 2-2015; 1-11
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