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dc.contributor.author
Zulian, Sandra Edith
dc.contributor.author
Ilincheta, Monica Graciela
dc.contributor.author
Giusto, Norma Maria
dc.date.available
2016-09-13T21:45:36Z
dc.date.issued
2010-12-15
dc.identifier.citation
Zulian, Sandra Edith; Ilincheta, Monica Graciela; Giusto, Norma Maria; Insulin modifies aging-related inhibition of 1-stearoyl, 2-achidonoylglycerol phosphorylation in rat synaptic terminals; Elsevier; Neurochemistry International; 58; 3; 15-12-2010; 330-336
dc.identifier.issn
0197-0186
dc.identifier.uri
http://hdl.handle.net/11336/7623
dc.description.abstract
The purpose of the present study was to analyze diacylglycerol kinase (DAGK) activity in synaptic terminals from cerebral cortex (CC) and hippocampus (Hp) from adult (3-4 month-old) and aged (26-28 month-old) rats. The effect of insulin through DAGK activity on synaptosomes from adult and aged rats was also analyzed under conditions favoring saturated or unsaturated phosphatidic acid (PA) formation, using exogenous di-palmitoil glycerol (DPG) or 1-stearoyl-2-arachidonoylglycerol (SAG) as substrates. Results showed that the enzymatic activity preferentially uses 1-stearoyl-2-arachidonoylglycerol (SAG) as substrate, thus indicating the presence of -type DAGK. A significant decrease in DAGK activity transforming SAG into PA was also observed in both tissues from aged rats. Western Blot detection of DAGK showed that enzyme content undergoes no changes with aging. [3H]inositol incorporation into phosphoinosites was also analyzed to evaluate the role of DAGK in their synthesis. Data obtained from 3H-inositol incorporation into phosphoinositides revealed that in synaptosomes from aged rats phosphatidylinositol (PI) synthesis is lower than in adult animals. Interestingly, in the presence of SAG, PI synthesis was restored to adult values. DAGK activity over SAG was more highly stimulated by insulin in CC and Hp synaptosomes of aged rats with respect to adult rats. On the other hand, insulin exerted a stimulatory effect on PI and phosphatidylinositol 4 phosphate (PI(4)P) synthesis in synaptosomal CC from aged rats. Taken together, our findings indicate that in aged rats insulin triggers a stimulatory mechanism that reverts the diminished synaptosomal ability to synthesize arachidonoyl phosphatidic acid (20:4 PA). The recovery of this PA species indicates that insulin positively regulates phosphoinositide synthesis.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Aging
dc.subject
Dagk
dc.subject
Synaptic Terminals
dc.subject
Dag
dc.subject
Insulin
dc.subject
Cerebral Cortex
dc.subject
Hippocampus
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Insulin modifies aging-related inhibition of 1-stearoyl, 2-achidonoylglycerol phosphorylation in rat synaptic terminals
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-07-05T18:47:42Z
dc.journal.volume
58
dc.journal.number
3
dc.journal.pagination
330-336
dc.journal.pais
Reino Unido
dc.journal.ciudad
Oxford
dc.description.fil
Fil: Zulian, Sandra Edith. Consejo Nacional de Investigaciones Cientificas y T__etilde__cnicas. Centro Cient__itilde__fico Tecnol__otilde__gico Bahia Blanca. Instituto de Investigaciones Bioqu__itilde__micas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
dc.description.fil
Fil: Ilincheta, Monica Graciela. Consejo Nacional de Investigaciones Cientificas y T__etilde__cnicas. Centro Cient__itilde__fico Tecnol__otilde__gico Bahia Blanca. Instituto de Investigaciones Bioqu__itilde__micas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
dc.description.fil
Fil: Giusto, Norma Maria. Consejo Nacional de Investigaciones Cientificas y T__etilde__cnicas. Centro Cient__itilde__fico Tecnol__otilde__gico Bahia Blanca. Instituto de Investigaciones Bioqu__itilde__micas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
dc.journal.title
Neurochemistry International
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.neuint.2010.12.005
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S019701861000375X
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