Artículo
Dissecting the Metal Selectivity of MerR Monovalent Metal Ion Sensors in Salmonella
Fecha de publicación:
07/2013
Editorial:
American Society For Microbiology
Revista:
Journal Of Bacteriology
ISSN:
0021-9193
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Two homologous transcription factors, CueR and GolS, that belong to the MerR metalloregulatory family are responsible for Salmonella Cu and Au sensing and resistance, respectively. They share similarities not only in their sequences, but also in their target transcription binding sites. While CueR responds similarly to Au, Ag, or Cu to induce the expression of its target genes, GolS shows higher activation by Au than by Ag or Cu. We showed that the ability of GolS to distinguish Au from Cu resides in the metal-binding loop motif. Here, we identify the amino acids within the motif that determine in vivo metal selectivity. We show that residues at positions 113 and 118 within the metal-binding loop are the main contributors to metal selectivity. The presence of a Pro residue at position 113 favors the detection of Cu, while the presence of Pro at position 118 disfavors it. Our results highlight the molecular bases that allow these regulators to coordinate the correct metal ion directing the response to a particular metal injury.
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Articulos(IBR)
Articulos de INST.DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Articulos de INST.DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Citación
Ibáñez, María Marta; Cerminati, Sebastián; Checa, Susana Karina; Soncini, Fernando Carlos; Dissecting the Metal Selectivity of MerR Monovalent Metal Ion Sensors in Salmonella; American Society For Microbiology; Journal Of Bacteriology; 195; 13; 7-2013; 3084-3092
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