Artículo
The gap gene giant of Rhodnius prolixus is maternally expressed and required for proper head and abdomen formation
Lavore, Andres Esteban
; Pagola, Lucia Elena
; Esponda Behrens, Natalia Irene
; Rivera Pomar, Rolando
Fecha de publicación:
01/2012
Editorial:
Academic Press Inc Elsevier Science
Revista:
Developmental Biology
ISSN:
0012-1606
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The segmentation process in insects depends on a hierarchical cascade of gene activity. The first effectors downstream of the maternal activation are the gap genes, which divide the embryo in broad fields. We discovered a sequence corresponding to the leucine-zipper domain of the orthologue of the gene giant (Rp- gt) in traces from the genome of Rhodnius prolixus, a hemipteran with intermediate germ-band development. We cloned the Rp- gt gene from a normalized cDNA library and characterized its expression and function. Bioinformatic analysis of 12.5. kbp of genomic sequence containing the Rp- gt transcriptional unit shows a cluster of bona fide regulatory binding sites, which is similar in location and structure to the predicted posterior expression domain of the Drosophila orthologue. Rp-gt is expressed in ovaries and maternally supplied in the early embryo. The maternal contribution forms a gradient of scattered patches of mRNA in the preblastoderm embryo. Zygotic Rp-gt is expressed in two domains that after germ band extension are restricted to the head and the posterior growth zone. Parental RNAi shows that Rp-gt is required for proper head and abdomen formation. The head lacks mandibulary and maxillary appendages and shows reduced clypeus-labrum, while the abdomen lacks anterior segments. We conclude that Rp-gt is a gap gene on the head and abdomen and, in addition, has a function in patterning the anterior head capsule suggesting that the function of gt in hemipterans is more similar to dipterans than expected. © 2011.
Palabras clave:
Gap Gene
,
Giant
,
Maternal Expression
,
Regulatory Cluster
,
Rhodnius
,
Segmentation
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Articulos(CCT - LA PLATA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Citación
Lavore, Andres Esteban; Pagola, Lucia Elena; Esponda Behrens, Natalia Irene; Rivera Pomar, Rolando; The gap gene giant of Rhodnius prolixus is maternally expressed and required for proper head and abdomen formation; Academic Press Inc Elsevier Science; Developmental Biology; 361; 1; 1-2012; 147-155
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