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dc.contributor.author
Martincová, Eva
dc.contributor.author
Voleman, Luboš
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Najdrová, Vladimíra
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de Napoli, Maximiliano Gabriel
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Eshar, Shiri
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Gualdron, Melisa
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Hopp, Christine S.
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Sanin, David E.
dc.contributor.author
Tembo, Dumizulu L.
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Van Tyne, Daria
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Walker, Dawn
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Marcinčiková, Michaela
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Tachezy, Jan
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Doležal, Pavel
dc.date.available
2019-05-09T16:22:30Z
dc.date.issued
2012-04
dc.identifier.citation
Martincová, Eva; Voleman, Luboš; Najdrová, Vladimíra; de Napoli, Maximiliano Gabriel; Eshar, Shiri; et al.; Live imaging of mitosomes and hydrogenosomes by HaloTag technology; Public Library of Science; Plos One; 7; 4; 4-2012; 1-5
dc.identifier.issn
1932-6203
dc.identifier.uri
http://hdl.handle.net/11336/75968
dc.description.abstract
Hydrogenosomes and mitosomes represent remarkable mitochondrial adaptations in the anaerobic parasitic protists such as Trichomonas vaginalis and Giardia intestinalis, respectively. In order to provide a tool to study these organelles in the live cells, the HaloTag was fused to G. intestinalis IscU and T. vaginalis frataxin and expressed in the mitosomes and hydrogenosomes, respectively. The incubation of the parasites with the fluorescent Halo-ligand resulted in highly specific organellar labeling, allowing live imaging of the organelles. With the array of available ligands the HaloTag technology offers a new tool to study the dynamics of mitochondria-related compartments as well as other cellular components in these intriguing unicellular eukaryotes.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Public Library of Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
Halo Tags
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Live Imaging
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Mitosomes
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Hydrogenosomes
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Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Live imaging of mitosomes and hydrogenosomes by HaloTag technology
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-05-06T13:33:10Z
dc.journal.volume
7
dc.journal.number
4
dc.journal.pagination
1-5
dc.journal.pais
Estados Unidos
dc.journal.ciudad
San Francisco
dc.description.fil
Fil: Martincová, Eva. Charles University; República Checa
dc.description.fil
Fil: Voleman, Luboš. Charles University; República Checa
dc.description.fil
Fil: Najdrová, Vladimíra. Charles University; República Checa
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Fil: de Napoli, Maximiliano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
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Fil: Eshar, Shiri. Hebrew University-Hadassah Medical School; Israel
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Fil: Gualdron, Melisa. Christian De Duve Institute of Cellular Pathology; Bélgica
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Fil: Hopp, Christine S.. School of Hygiene and Tropical Medicine. Malaria Centre; Reino Unido
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Fil: Sanin, David E.. University of York; Reino Unido
dc.description.fil
Fil: Tembo, Dumizulu L.. Malawi-Liverpool Wellcome Trust Clinical Research Programme; Malaui
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Fil: Van Tyne, Daria. Harvard University. Harvard School of Public Health; Estados Unidos
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Fil: Walker, Dawn. Integrated Biomedical Sciences Center for Microbial Interface Biology Department of Internal Medicine; Estados Unidos
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Fil: Marcinčiková, Michaela. Charles University; República Checa
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Fil: Tachezy, Jan. Charles University; República Checa
dc.description.fil
Fil: Doležal, Pavel. Charles University; República Checa
dc.journal.title
Plos One
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1371/journal.pone.0036314
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0036314
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