Artículo
Determination of Reactive Oxygen Species in Salt-Stressed Plant Tissues
Fecha de publicación:
08/2012
Editorial:
Humana Press
Revista:
Methods in Molecular Biology
ISSN:
1064-3745
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Reactive oxygen species (ROS) participate in signaling events that regulate ion channel activity and gene expression. However, excess ROS exert adverse effects that stem from their interaction with macromolecules. Thus, the assessment of the effects of salinity on ROS changes are central to understanding how plants respond and cope with this stress. ROS determination in salt-stressed plants poses specific challenges. On the one hand, salinity comprises osmotic and ion-specific effects which may, in turn, have different effects on ROS production. On the other hand, changes in ROS production may happen when tissues from salinized plants are subject to water potential (Ψ) changes when incubated in non-isosmotic solutions. This chapter provides detailed accounts of methods for ROS detection in tissues from salt-stressed plants and includes suggestions for avoiding artifacts when dealing with such tissues.
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Identificadores
Colecciones
Articulos(CCT - LA PLATA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos(IIB-INTECH)
Articulos de INST.DE INVEST.BIOTECNOLOGICAS - INSTITUTO TECNOLOGICO CHASCOMUS
Articulos de INST.DE INVEST.BIOTECNOLOGICAS - INSTITUTO TECNOLOGICO CHASCOMUS
Citación
Rodriguez, Andres Alberto; Taleisnik, Edith; Determination of Reactive Oxygen Species in Salt-Stressed Plant Tissues; Humana Press; Methods in Molecular Biology; 913; 8-2012; 225-236
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