Artículo
Isolation and molecular characterization of a new Neospora caninum isolate from cattle in Argentina
Campero, Lucía María
; Venturini, María Cecilia; Moore, Dadin Prando
; Massola, L.; Lagomarsino, H.; Garcia, B.; Bacigalupe, Diana; Rambeaud, Magdalena
; Pardini, Lais Luján
; Leunda, Maria Rosa; Schares, G; Campero, Carlos Manuel
Fecha de publicación:
08/2015
Editorial:
Academic Press Inc Elsevier Science
Revista:
Experimental Parasitology
ISSN:
0014-4894
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Neospora caninum is one of the most important causes of bovine abortion, but isolation of live parasites from infected tissue is difficult. The aims of the present study were to obtain new isolates of N. caninum from congenitally infected asymptomatic newborn cattle in Argentina and to perform characterization by multilocus-microsatellite analysis. Five clinically normal born calves, with demonstrable N. caninum antibodies in precolostrum serum by indirect fluorescent antibody test, were euthanized and their brain samples were processed for histopathological, immunohistochemical, polymerase chain reaction (PCR) analysis, and for bioassay in γ-interferon knockout (GKO) mice. Although N. caninum DNA was detected in brain from all the calves by PCR, viable N. caninum was isolated in GKO mice from only one calf. Neospora caninum tachyzoites of this Argentinean isolate, designated NC-Argentina LP1, were propagated in VERO cell cultures seeded with tachyzoites from the infected GKO mice tissues. Multilocus-microsatellite typing on DNA derived from cell cultured tachyzoites revealed a unique genetic pattern, different from reported isolates. This is the first bovine isolation and genetic characterization of N. caninum in Argentina.
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Licencia
Identificadores
Colecciones
Articulos(CCT - LA PLATA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos(CCT - MAR DEL PLATA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - MAR DEL PLATA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - MAR DEL PLATA
Citación
Campero, Lucía María; Venturini, María Cecilia; Moore, Dadin Prando; Massola, L.; Lagomarsino, H.; et al.; Isolation and molecular characterization of a new Neospora caninum isolate from cattle in Argentina; Academic Press Inc Elsevier Science; Experimental Parasitology; 155; 8-2015; 8-12
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