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dc.contributor.author
Naum Ongania, Gabriela
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dc.contributor.author
Díaz Cortés, Víctor Manuel
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dc.contributor.author
Blasi, Francesco
dc.contributor.author
Rivera Pomar, Rolando
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dc.date.available
2019-05-07T22:41:25Z
dc.date.issued
2014-01-06
dc.identifier.citation
Naum Ongania, Gabriela; Díaz Cortés, Víctor Manuel; Blasi, Francesco; Rivera Pomar, Rolando; Nuclear actin polymerization from faster growing ends in the initial activation of hox gene transcription: Are nuclear speckles involved?; Taylor and Francis Inc.; Transcription; 4; 5; 6-1-2014; 260-272
dc.identifier.issn
1949-1034
dc.identifier.uri
http://hdl.handle.net/11336/75838
dc.description.abstract
The HoxB cluster expression is activated by retinoic acid and transcribed in a collinear manner. The DNA-binding Pknox1-Pbx1 complex modulates Hox protein activity. Here, NT2-D1 teratocarcinoma cells -a model of Hox gene expression- were used to show that upon retinoic acid induction, Pknox1 co-localizes with polymeric nuclear actin. We have found that globular actin aggregates, polymeric actin, the elongating RNA polymerase II and THOC match euchromatic regions corresponding to nuclear speckles. Moreover, RNA polymerase II, N-WAS P, and transcription/splicing factors p54nrb and PSF were validated as Pknox1 interactors by tandem affinity purification. PSF pulled down with THOC and nuclear actin, both of which co-localize in nuclear speckles. Although latrunculin A slightly decreases the general level of HoxB gene expression, inhibition of nuclear actin polymerization by cytochalasin D blocks the expression of HoxB transcripts in a collinear manner. Thus, our results support the hypothesis that nuclear actin polymerization is involved in the activation of HoxB gene expression by means of nuclear speckles. © 2013 Landes Bioscience.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Taylor and Francis Inc.
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Co-Transcriptional Splicing
dc.subject
Hox Genes
dc.subject
Nuclear Actin
dc.subject
Nuclear Speckles
dc.subject
Rna Pol Ii
dc.subject
Transcription/Splicing
dc.subject.classification
Genética y Herencia
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Nuclear actin polymerization from faster growing ends in the initial activation of hox gene transcription: Are nuclear speckles involved?
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-05-06T13:40:50Z
dc.journal.volume
4
dc.journal.number
5
dc.journal.pagination
260-272
dc.journal.pais
Estados Unidos
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dc.description.fil
Fil: Naum Ongania, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina
dc.description.fil
Fil: Díaz Cortés, Víctor Manuel. Institut Municipal d’Investigació Medica (IMIM); Hospital del Mar, Barcelona; España
dc.description.fil
Fil: Blasi, Francesco. Istituto FIRC di Oncologia Molecolare (IFOM); Milan; Italia
dc.description.fil
Fil: Rivera Pomar, Rolando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina
dc.journal.title
Transcription
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.4161/trns.27672
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.4161/trns.27672
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