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dc.contributor.author
Acardi, Soraya Alejandra  
dc.contributor.author
Rago, María Virginia  
dc.contributor.author
Liotta, Domingo Javier  
dc.contributor.author
Fernandez Duque, Eduardo  
dc.contributor.author
Salomon, Oscar Daniel  
dc.date.available
2016-09-08T14:52:44Z  
dc.date.issued
2013-03  
dc.identifier.citation
Acardi, Soraya Alejandra; Rago, María Virginia; Liotta, Domingo Javier; Fernandez Duque, Eduardo; Salomon, Oscar Daniel; Leishmania (Viannia) DNA detection by PCR-RFLP and sequencing in free-ranging owl monkeys (Aotus azarai azarai) from Formosa, Argentina; Elsevier Science; Veterinary Parasitology; 193; 1-3; 3-2013; 256-259  
dc.identifier.issn
0304-4017  
dc.identifier.uri
http://hdl.handle.net/11336/7554  
dc.description.abstract
American Cutaneous Leishmaniasis (ACL) is caused by protozoan parasites of the Leishmania genus, and transmitted by females of the Phlebotominae family. The role of wild and domestic hosts in the cycle of Leishmania is still unknown. ACL is endemic in the province of Formosa where Nyssomyia neivai was the most abundant species in several captures and 31 cumulative ACL human cases were reported between 2005 and 2011 in the province. The present report describes the detection, by PCR-RFLP and confirmed by sequencing, of subgenus Leishmania (Viannia) DNA in four free-ranging owl monkeys (Aotus azarai azarai) from Formosa Province. The sequence amplified was the mini-exon gene present in tandem repeats in all species of the Leishmania genus from buffy coat samples. The absence of inhibitors in the samples was checked by a β-globin protocol originally designed to amplify the human β-globin gene. However, other free-ranging primates were found with natural infections of L. (V) braziliensis complex and Leishmania (Viannia) subgenus by parasitological means in America. To the best of our knowledge, there are no published reports on detection of subgenus Leishmania (Viannia) DNA by PCR-RFLP in argentinean free-ranging primates. Additional eco-epidemiological and parasitological studies are necessary to confirm owl monkeys, or any other natural infected mammal species detected by PCR, as a reservoir, incidental host or to propose it as an animal model for research on this topic.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier Science  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/  
dc.subject
Leishmania  
dc.subject
Dna  
dc.subject
Primates  
dc.subject.classification
Biología Celular, Microbiología  
dc.subject.classification
Ciencias Biológicas  
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS  
dc.title
Leishmania (Viannia) DNA detection by PCR-RFLP and sequencing in free-ranging owl monkeys (Aotus azarai azarai) from Formosa, Argentina  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2016-09-02T18:06:28Z  
dc.journal.volume
193  
dc.journal.number
1-3  
dc.journal.pagination
256-259  
dc.journal.pais
Países Bajos  
dc.journal.ciudad
Amsterdam  
dc.description.fil
Fil: Acardi, Soraya Alejandra. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina. Ministerio de Salud. Instituto Nacional de Medicina Tropical; Argentina  
dc.description.fil
Fil: Rago, María Virginia. Wildlife Conservation Society; Estados Unidos  
dc.description.fil
Fil: Liotta, Domingo Javier. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina. Ministerio de Salud. Instituto Nacional de Medicina Tropical; Argentina  
dc.description.fil
Fil: Fernandez Duque, Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Centro de Ecologia Aplicada del Litoral (i); Argentina. University of Pennsylvania; Estados Unidos  
dc.description.fil
Fil: Salomon, Oscar Daniel. Instituto Nacional de Medicina Tropical, Misiones; Argentina  
dc.journal.title
Veterinary Parasitology  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0304401712006607  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.vetpar.2012.12.012