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dc.contributor.author
Gómez Lobato, María Eugenia
dc.contributor.author
Civello, Pedro Marcos
dc.contributor.author
Martinez, Gustavo Adolfo
dc.date.available
2019-04-29T19:54:16Z
dc.date.issued
2011-07-06
dc.identifier.citation
Gómez Lobato, María Eugenia; Civello, Pedro Marcos; Martinez, Gustavo Adolfo; Effects of ethylene, cytokinin, and physical treatments on BoPaO gene expression of harvested broccoli.; John Wiley & Sons Ltd; Journal of the Science of Food and Agriculture; 92; 1; 6-7-2011; 151-158
dc.identifier.issn
0022-5142
dc.identifier.uri
http://hdl.handle.net/11336/75246
dc.description.abstract
BACKGROUND: Broccoli is a highly perishable vegetable that shows enhanced postharvest senescence and intense de‐greening caused by chlorophyll degradation. One of the key steps of chlorophyll catabolism is the opening of chlorophyll tretrapyrrole catalysed by pheophorbide a oxygenase (PaO). In this study the expression of a gene encoding a putative PaO was characterised under several chemical and physical treatments.
RESULTS: A fragment of a gene encoding a PaO from broccoli (BoPaO) was cloned. The expression of BoPaO showed an important increment during postharvest senescence, in correlation with chlorophyll degradation. Furthermore, broccoli heads were treated with the hormones cytokinin and ethylene. Cytokinin delayed the increment in BoPaO expression, while ethylene accelerated the process. Also, several postharvest treatments were applied in order to evaluate their effect on BoPaO expression. Samples treated with modified atmosphere, hot air, UV‐C or white light showed a delay in chlorophyll degradation and de‐greening. In most cases the treatments also delayed the increment in BoPaO expression during senescence.
CONCLUSION: A close correlation between chlorophyll degradation and BoPaO expression was found during broccoli senescence. This relationship was corroborated in samples treated with different hormonal and physical applications. Copyright © 2011 Society of Chemical Industry
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
John Wiley & Sons Ltd
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Broccoli
dc.subject
Senescence
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Chlorophyll Degradation
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Pheophorbide a Oxygenase
dc.subject.classification
Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Effects of ethylene, cytokinin, and physical treatments on BoPaO gene expression of harvested broccoli.
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-04-26T18:25:35Z
dc.identifier.eissn
1097-0010
dc.journal.volume
92
dc.journal.number
1
dc.journal.pagination
151-158
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Gómez Lobato, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
dc.description.fil
Fil: Civello, Pedro Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
dc.description.fil
Fil: Martinez, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
dc.journal.title
Journal of the Science of Food and Agriculture
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/jsfa.4555
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/jsfa.4555/pdf
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