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dc.contributor.author Rodríguez Durán, Luis
dc.contributor.author Spelzini, Darío
dc.contributor.author Boeris, Valeria
dc.contributor.author Cristobal Aguilar, N.
dc.contributor.author Picó, Guillermo Alfredo
dc.date.available 2016-09-06T15:18:12Z
dc.date.issued 2013-01
dc.identifier.citation Rodríguez Durán, Luis; Spelzini, Darío; Boeris, Valeria; Cristobal Aguilar, N.; Picó, Guillermo Alfredo; Partition in aqueous two-phase system: its application in downstream processing of tannase from Aspergillus niger; Elsevier; Colloids And Surfaces B: Biointerfaces; 101; 1-2013; 392-397
dc.identifier.issn 0927-7765
dc.identifier.uri http://hdl.handle.net/11336/7469
dc.description.abstract Tannase from Aspergillus niger was partitioned in aqueous two-phase systems composed by polyethyleneglycol of molar mass 400, 600 and 1000 and potassium phosphate. Tannase was found to be partitioned toward the salt-rich phase in all systems, with partition coefficients lower than 0.5. Partition coefficients values and low entropic and enthalpic changes associated with tannase partition suggest that the entropic effect may be the driving force of the concentration of the enzyme in the bottom phase due to the high molar mass of the enzyme. The process was significantly influenced by the top phase/bottom phase volume ratio. When the fungal culture broth was partitioned in these systems, a good performance was found, since the enzyme recovery in the bottom phase of the system composed by polyethyleneglycol 1000 was around 96% with a 7.0-fold increase in purity.
dc.format application/pdf
dc.language.iso eng
dc.publisher Elsevier
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject AQUEOUS TWO PHASE SYSTEM
dc.subject TANNASE
dc.subject PARTITION
dc.subject PURIFICATION
dc.subject INTERACTION
dc.subject DOWNSTREAM PROCESSING
dc.subject.classification Físico-Química, Ciencia de los Polímeros, Electroquímica
dc.subject.classification Ciencias Químicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Partition in aqueous two-phase system: its application in downstream processing of tannase from Aspergillus niger
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2016-06-01T13:51:32Z
dc.journal.volume 101
dc.journal.pagination 392-397
dc.journal.pais Países Bajos
dc.journal.ciudad Amsterdam
dc.description.fil Fil: Rodríguez Durán, Luis. Universidad Autónoma de Coahuila; México
dc.description.fil Fil: Spelzini, Darío. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Quimica y Fisica. Area Fisicoquimica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; Argentina
dc.description.fil Fil: Boeris, Valeria. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Quimica y Fisica. Area Fisicoquimica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; Argentina
dc.description.fil Fil: Cristobal Aguilar, N.. Universidad Autónoma de Coahuila; México
dc.description.fil Fil: Picó, Guillermo Alfredo. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Quimica y Fisica. Area Fisicoquimica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; Argentina
dc.journal.title Colloids And Surfaces B: Biointerfaces
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0927776512004171
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.colsurfb.2012.07.023


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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Atribución-NoComercial-SinDerivadas 2.5 Argentina (CC BY-NC-ND 2.5 AR)