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dc.contributor.author
Rodríguez Durán, Luis
dc.contributor.author
Spelzini, Darío
dc.contributor.author
Boeris, Valeria
dc.contributor.author
Cristobal Aguilar, N.
dc.contributor.author
Picó, Guillermo Alfredo
dc.date.available
2016-09-06T15:18:12Z
dc.date.issued
2013-01
dc.identifier.citation
Rodríguez Durán, Luis; Spelzini, Darío; Boeris, Valeria; Cristobal Aguilar, N.; Picó, Guillermo Alfredo; Partition in aqueous two-phase system: its application in downstream processing of tannase from Aspergillus niger; Elsevier; Colloids And Surfaces B: Biointerfaces; 101; 1-2013; 392-397
dc.identifier.issn
0927-7765
dc.identifier.uri
http://hdl.handle.net/11336/7469
dc.description.abstract
Tannase from Aspergillus niger was partitioned in aqueous two-phase systems composed by polyethyleneglycol of molar mass 400, 600 and 1000 and potassium phosphate. Tannase was found to be partitioned toward the salt-rich phase in all systems, with partition coefficients lower than 0.5. Partition coefficients values and low entropic and enthalpic changes associated with tannase partition suggest that the entropic effect may be the driving force of the concentration of the enzyme in the bottom phase due to the high molar mass of the enzyme. The process was significantly influenced by the top phase/bottom phase volume ratio. When the fungal culture broth was partitioned in these systems, a good performance was found, since the enzyme recovery in the bottom phase of the system composed by polyethyleneglycol 1000 was around 96% with a 7.0-fold increase in purity.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Aqueous Two Phase System
dc.subject
Tannase
dc.subject
Partition
dc.subject
Purification
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Interaction
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Downstream Processing
dc.subject.classification
Físico-Química, Ciencia de los Polímeros, Electroquímica
dc.subject.classification
Ciencias Químicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Partition in aqueous two-phase system: its application in downstream processing of tannase from Aspergillus niger
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-06-01T13:51:32Z
dc.journal.volume
101
dc.journal.pagination
392-397
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Rodríguez Durán, Luis. Universidad Autónoma de Coahuila; México
dc.description.fil
Fil: Spelzini, Darío. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Quimica y Fisica. Area Fisicoquimica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; Argentina
dc.description.fil
Fil: Boeris, Valeria. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Quimica y Fisica. Area Fisicoquimica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; Argentina
dc.description.fil
Fil: Cristobal Aguilar, N.. Universidad Autónoma de Coahuila; México
dc.description.fil
Fil: Picó, Guillermo Alfredo. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Quimica y Fisica. Area Fisicoquimica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; Argentina
dc.journal.title
Colloids And Surfaces B: Biointerfaces
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0927776512004171
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.colsurfb.2012.07.023


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