Mostrar el registro sencillo del ítem
dc.contributor.author
Blanco, Nicolás Ernesto
dc.contributor.author
Ceccoli, Romina Denis
dc.contributor.author
Dalla Via, Maria Virginia
dc.contributor.author
Voss, Ingo
dc.contributor.author
Segretin, Maria Eugenia
dc.contributor.author
Bravo Almonacid, Fernando Felix
dc.contributor.author
Melzer, Michael
dc.contributor.author
Hajirezaei, Mohammad Reza
dc.contributor.author
Scheibe, Renate
dc.contributor.author
Hanke, Guy T.
dc.date.available
2016-09-06T15:14:42Z
dc.date.issued
2013-02
dc.identifier.citation
Blanco, Nicolás Ernesto; Ceccoli, Romina Denis; Dalla Via, Maria Virginia; Voss, Ingo; Segretin, Maria Eugenia; et al.; Expression of the minor isoform pea ferredoxin in tobacco alters photosynthetic electron partitioning and enhances cyclic electron flow; American Society Of Plant Biologist; Plant Physiology.; 161; 2; 2-2013; 866-879
dc.identifier.issn
0032-0889
dc.identifier.uri
http://hdl.handle.net/11336/7466
dc.description.abstract
Ferredoxins (Fds) are ferrosulfoproteins that function as low-potential electron carriers in plants. The Fd family is composed of several isoforms that share high sequence homology but differ in functional characteristics. In leaves, at least two isoforms conduct linear and cyclic photosynthetic electron transport around photosystem I, and mounting evidence suggests the existence of at least partial division of duties between these isoforms. To evaluate the contribution of different kinds of Fds to the control of electron fluxes along the photosynthetic electron transport chain, we overexpressed a minor pea (Pisum sativum) Fd isoform (PsFd1) in tobacco (Nicotiana tabacum) plants. The transplastomic OeFd1 plants exhibited variegated leaves and retarded growth and developmental rates. Photosynthetic studies of these plants indicated a reduction in carbon dioxide assimilation rates, photosystem II photochemistry, and linear electron flow. However, the plants showed an increase in nonphotochemical quenching, better control of excitation pressure at photosystem II, and no evidence of photoinhibition, implying a better dynamic regulation to remove excess energy from the photosynthetic electron transport chain. Finally, analysis of P700 redox status during illumination confirmed that the minor pea Fd isoform promotes enhanced cyclic flow around photosystem I. The two novel features of this work are: (1) that Fd levels achieved in transplastomic plants promote an alternative electron partitioning even under greenhouse light growth conditions, a situation that is exacerbated at higher light intensity measurements; and (2) that an alternative, minor Fd isoform has been overexpressed in plants, giving new evidence of labor division among Fd isoforms.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Society Of Plant Biologist
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Ferredoxin
dc.subject
Overexpressor
dc.subject
Transplastomic
dc.subject
Tobacco
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Expression of the minor isoform pea ferredoxin in tobacco alters photosynthetic electron partitioning and enhances cyclic electron flow
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-02-01T16:38:00Z
dc.journal.volume
161
dc.journal.number
2
dc.journal.pagination
866-879
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Rockville
dc.description.fil
Fil: Blanco, Nicolás Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad de Umea; Suecia
dc.description.fil
Fil: Ceccoli, Romina Denis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
dc.description.fil
Fil: Dalla Via, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; Argentina
dc.description.fil
Fil: Voss, Ingo. Universität Osnabrück; Alemania
dc.description.fil
Fil: Segretin, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones En Ingeniería Genética y Biología Molecular; Argentina
dc.description.fil
Fil: Bravo Almonacid, Fernando Felix. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones En Ingeniería Genética y Biología Molecular; Argentina
dc.description.fil
Fil: Melzer, Michael. Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung, Physiologie und Zellbiologie; Alemania
dc.description.fil
Fil: Hajirezaei, Mohammad Reza. Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung, Physiologie und Zellbiologie; Argentina
dc.description.fil
Fil: Scheibe, Renate. Universität Osnabrück; Alemania
dc.description.fil
Fil: Hanke, Guy T.. Universität Osnabrück; Alemania
dc.journal.title
Plant Physiology.
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.plantphysiol.org/content/161/2/866
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1104/pp.112.211078
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3561025/
Archivos asociados