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Artículo

Increased cellular uptake of lauryl gallate loaded in superparamagnetic poly(methyl methacrylate) nanoparticles due to surface modification with folic acid

Feuser, Paulo Emilio; Carpio Arévalo, Juan Marcelo; Lima, Enio JuniorIcon ; Rodrigues Rossi, Gustavo; Trindade, Edvaldo da Silva; Merlin Rocha, Maria Eliane; Virtuoso Jacques, Amanda; Ricci Júnior, Eduardo; Santos Silva, Maria Claudia; Sayer, Claudia; Hermes de Araújo, Pedro H.
Fecha de publicación: 12/2016
Editorial: Springer
Revista: Journal of Materials Science: Materials In Medicine
ISSN: 0957-4530
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ingeniería Química

Resumen

Lauryl gallate loaded in superparamagnetic poly (methyl methacrylate) nanoparticles surface modified with folic acid were synthesized by miniemulsion polymerization in just one step. In vitro biocompatibility and cytotoxicity assays on L929 (murine fibroblast), human red blood, and HeLa (uterine colon cancer) cells were performed. The effect of folic acid at the nanoparticles surface was evaluated through cellular uptake assays in HeLa cells. Resultsshowed that the presence of folic acid did not affect substantially the polymer particle size (~120 nm), the superparamagnetic behavior, the encapsulation efficiency of lauryl gallate (~87 %), the Zeta potential (~38 mV) of thepolymeric nanoparticles or the release profile of lauryl gallate. The release profile of lauryl gallate from superparamagnetic poly(methyl methacrylate) nanoparticles presented an initial burst effect (0?1 h) followed by a slow andsustained release, indicating a biphasic release system.Lauryl gallate loaded in superparamagnetic poly(methyl methacrylate) nanoparticles with folic acid did not present cytotoxicity effects on L929 and human red blood cells. However, free lauryl gallate presented significant cytotoxic effects on L929 and human red blood cells at all tested concentrations. The presence of folic acid increased the cytotoxicity of lauryl gallate loaded in nanoparticles on HeLa cells due to a higher cellular uptake when HeLa cells were incubated at 37 °C. On the other hand, when the nanoparticles were incubated at low temperature (4 °C) cellular uptake was not observed, suggesting that the uptake occurred by folate receptor mediated energy-dependent endocytosis. Based on presented results our work suggeststhat this carrier system can be an excellent alternative in targeted drug delivery by folate receptor.
Palabras clave: Polimeric Nanoparticles , Superparamagnetism
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution 2.5 Unported (CC BY 2.5)
Identificadores
URI: http://hdl.handle.net/11336/74376
DOI: http://dx.doi.org/10.1007/s10856-016-5796-0
URL: https://link.springer.com/article/10.1007%2Fs10856-016-5796-0
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Articulos(CCT - PATAGONIA NORTE)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - PATAGONIA NORTE
Citación
Feuser, Paulo Emilio; Carpio Arévalo, Juan Marcelo; Lima, Enio Junior; Rodrigues Rossi, Gustavo; Trindade, Edvaldo da Silva; et al.; Increased cellular uptake of lauryl gallate loaded in superparamagnetic poly(methyl methacrylate) nanoparticles due to surface modification with folic acid; Springer; Journal of Materials Science: Materials In Medicine; 27; 12-2016; 185-200
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